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Escherichia coli enterotoxin II signal peptide variant and microorganism expressing fusion protein of the variant and heterologous protein
Escherichia coli enterotoxin II signal peptide variant and microorganism expressing fusion protein of the variant and heterologous protein
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机译：大肠杆菌肠毒素II信号肽变体和表达该变体与异源蛋白融合蛋白的微生物
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摘要
A heterologous protein is produced by: (i) culturing a microorganism transformed with an expression vector comprising a gene encoding a modified E. coli enterotoxin II signal peptide fused with the heterologous protein to produce and secrete the heterologous protein to periplasm, the modified E. coli enterotoxin II signal peptide being obtained by replacing at least one of the 2nd, 4th, 5th, 12th, 20th, and 22nd amino acids of E. coli enterotoxin II signal peptide of the following amino acid sequence (SEQ ID NO: 1) with another amino acid, with the proviso that at least one of the 2nd and 4th amino acid of the modified peptide is lysine; and (ii) recovering the heterologous protein from the periplasm.
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机译：异源蛋白的产生方法是：（i）培养一种用表达载体转化的微生物，该表达载体包含编码与异源蛋白融合的修饰的大肠杆菌肠毒素II信号肽的基因，以产生异种蛋白并将其分泌到周质中，即修饰的E.通过用以下氨基酸序列（SEQ ID NO：1）的大肠杆菌肠毒素II信号肽的第2、4、5、12、20和22位氨基酸中的至少一个取代获得大肠杆菌肠毒素II信号肽。另一个氨基酸，条件是修饰的肽的第二和第四氨基酸中的至少一个是赖氨酸; （ii）从周质中回收异源蛋白。
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