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Determining specificity and activity of human T cells, useful e.g. for diagnosing infections, comprises enzyme-linked immunospot assay with the antigen provided as peptide library
Determining specificity and activity of human T cells, useful e.g. for diagnosing infections, comprises enzyme-linked immunospot assay with the antigen provided as peptide library
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机译:确定人T细胞的特异性和活性,例如用于诊断感染,包括酶联免疫斑点测定,抗原作为肽库提供
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摘要
Quantitative determination of the specificity and activity of T cells in a human test material (e.g. blood) is based on the ELISPOT (enzyme-linked immunospot) assay, where the antigen required for reactivity of the cells is provided in the reaction mixture by multiple overlapping peptides (I) of 5-20 amino acids (a peptide library) and the T cells are specific for viruses, bacteria, protozoa, fungi, tumors or self-structures. An Independent claim is also included for an ELISPOT kit for this method.
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