首页> 外国专利> A method for immunologically - functional detection of biologically active insulin - like growth factor binding proteins or soluble insulin - like growth factor - receptors in the immunoassay

A method for immunologically - functional detection of biologically active insulin - like growth factor binding proteins or soluble insulin - like growth factor - receptors in the immunoassay

机译:一种在免疫测定中免疫功能检测生物活性胰岛素样生长因子结合蛋白或可溶性胰岛素样生长因子受体的方法。

摘要

A method for immunologically - functional detection of biologically active insulin - like growth factor binding proteins or soluble insulin - like growth factor - receptors in the immunoassay, comprising the steps of: dollars a 1.) Separation of the binding prot one of the sample with a specific antibody. Dollars a 2.) Functional detection of the separated binding prot of a by means of a labelled ligand. Dollars a method according to claim 1, characterized in that the ligand is an insulin - like growth factor or derived from this derivative or a partial sequence, the at least 3 amino acids of the natural sequences, or a peptide or a different synthetic ligand, which the structure or bonding capability of the insulin - like growth factor or partial sequences of insulin - like growth factor stated, is. Dollars a method according to claim 1, characterised in that markings are all the marks, via which a direct or indirect detection of the to the insulin - like growth factor binding proteins or the soluble receptors can be bonded ligands, for example, as a direct or indirect detection by means of measurements of the radioactivity of the enzymatic activity, the chemical - and bioluminescence, of the fluorescence.
机译:一种在免疫测定中对生物活性胰岛素样生长因子结合蛋白或可溶性胰岛素样生长因子受体进行免疫功能检测的方法,包括以下步骤:a。)分离样品的结合蛋白特异性抗体。 a。)通过标记的配体对分离的a的结合蛋白的功能检测。 2.根据权利要求1所述的方法,其特征在于,所述配体是胰岛素样生长因子或衍生自该衍生物或部分序列,天然序列的至少3个氨基酸或肽或不同的合成配体,所述的胰岛素(如生长因子)或胰岛素的部分序列(如生长因子)的结构或结合能力是什么。 2.根据权利要求1所述的方法,其特征在于,所述标记是所有标记,通过所述标记,可以直接或间接检测对胰岛素样生长因子结合蛋白或可溶性受体的结合配体,例如,作为直接结合的配体。或通过测量酶的放射性,荧光的化学发光和生物发光来间接检测。

著录项

  • 公开/公告号DE19719001C2

    专利类型

  • 公开/公告日2001-05-10

    原文格式PDF

  • 申请/专利权人 SCHÜTZDELLER ANGELIKA 72074 TÜBINGEN DE;

    申请/专利号DE1997119001

  • 发明设计人

    申请日1997-05-06

  • 分类号G01N33/566;G01N33/58;G01N33/68;

  • 国家 DE

  • 入库时间 2022-08-22 01:10:38

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