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Double-stranded dna with cohesive end(s), and method of shuffling dna using the same

机译:具有粘性末端的双链dna及其改组dna的方法

摘要

To provide a method of mutation of DNAs, which is substantially different from the conventional methods applicable to naturally-existing DNAs, and also to provide useful genetic products to be produced by the use of thus-mutated DNAs. A DNA with a cohesive end comprising (a) a double-stranded DNA having the same sequence as that of a part of a gene, and (b) a single-stranded DNA having a base sequence that exists on said gene at the site not adjoining the part corresponding to said double-stranded DNA or a base sequence which said gene does not have, wherein the single-stranded DNA is linked to either one end of the double-stranded DNA to form a cohesive end; a method for producing it; a method of shuffling a DNA using it; a DNA and a DNA pool to be obtained by the shuffling method; a method for producing the DNA pool; and a genetic product to be obtained by expressing the genetic information existing in the DNA pool.
机译:提供一种DNA的突变方法,该方法与适用于天然存在的DNA的常规方法大不相同,并且还提供了通过使用如此突变的DNA产生的有用的遗传产物。具有粘性末端的DNA,其包含(a)具有与基因的一部分相同的序列的双链DNA,和(b)具有在所述基因上不存在该位点的碱基序列的单链DNA。邻接对应于所述双链DNA或所述基因不具有的碱基序列的部分,其中所述单链DNA连接至所述双链DNA的任一端以形成内聚端;一种生产方法;用DNA改组DNA的方法;通过改组方法获得的DNA和DNA库;用于产生DNA库的方法;通过表达存在于DNA库中的遗传信息而获得的遗传产物。

著录项

  • 公开/公告号AU3691797A

    专利类型

  • 公开/公告日1998-02-25

    原文格式PDF

  • 申请/专利权人 NOVO-NORDISK A/S;

    申请/专利号AU19970036917

  • 发明设计人 YOSHIAKI MIYOTA;SHIRO FUKUYAMA;

    申请日1997-07-23

  • 分类号C12N15/10;C12Q1/68;C12N9/00;

  • 国家 AU

  • 入库时间 2022-08-22 02:53:25

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