首页>
外国专利>
METHOD OF CELL-FREE IN VITRO TRANSCRIBING A DNA MATRIX, DNA MATRIX A SUCH AND APPLICATION OF THAT METHOD AND SUCH DNA MATRIX
METHOD OF CELL-FREE IN VITRO TRANSCRIBING A DNA MATRIX, DNA MATRIX A SUCH AND APPLICATION OF THAT METHOD AND SUCH DNA MATRIX
展开▼
机译：DNA基质，DNA基质A的体外无细胞翻译方法及其应用和这种DNA基质的应用
展开▼
页面导航
摘要
著录项
相似文献
摘要
Process for transcription of a DNA template in vitro in a cell-free system, where the template comprises a sequence to be transcribed under the control of at least one regulatory element, is characterised in that (a) an enriched and optionally purified extract of cell nuclei and at least one labelled nucleotide are used for the transcription, where the extract can optionally be supplemented or partly or completely replaced by transcription factors and/or cofactors, (b) proteins present in the reaction mixture at the end of transcription are optionally separated and/or degraded, (c) the labelled transcript is bound to a solid support, (d) excess labelled nucleotide is removed, and (e) the amount of labelled transcript is determined. Also claimed is a DNA template as above in which the sequence to be transcribed does not contain at least one nucleobase selected from G, C or T, where the G-, C- or T-free sequence has a length of more than 400 nucleotides.
展开▼
