摘要：
Objective To explore the normal range of plasma VWF levels of healthy Chinese and to analyze the influencingtactors to VWF level.Methods To detect the levels of von Willebrand factor antigen (VWF ∶ Ag),von Willebrand factor ristocetin cofactor activity (VWF ∶ Rco),von Willebrand factor collagen binding activity (VWF∶ CB),and the factor Ⅷ coagulation activity (F Ⅷ ∶ C) by using fully automatic and standardized testing instruments and matching reagent in 70 healthy Chinese.The effects of age,ABO blood type,gender and region were also analyzed.Meanwhile,8 standard plasma samples (2 normal subjects,6 cases of type 2 VWD) confirmed by NIBSC were tested for VWF values.Results ① In 70 cases of healthy Chinese,the mean value of plasma VWF ∶ Ag,VWF ∶ Rco and VWF ∶ CB wvere (95.4± 44.9)％,(105.9±35.4)％ and (89.8±28.4)％,respectively;the ratio of VWF∶ Rco/VWF∶Ag and VWF∶CB/ VWF∶ Ag was 1.18±0.25 and 1.03±0.29,respectively.③There was no statistical significance in plasma VWF values between the age ≥30 years and ＜30 years group (P＞0.05).③The VWF∶ Rco,VWF∶ CB of type O blood group were lower than that of non-O group (t=2.074,P=0.042;t=3.949,P=0.001),but there was no statistical significance in VWF ∶ Ag,VWF ∶ Rco/VWF ∶ Ag,VWF ∶ CB/VWF ∶ Ag between the two groups (P＞0.05).④There was no significant difference in VWF values between male and female groups (P＞0.05).⑤The VWF∶ Ag,VWF∶ CB of the northern population (North area of Huaihe River) group were higher than that of southern population (Suzhou area) group (t=4.525,P=0.001;r=3.214,P=0.002),but VWF ∶ Rco/VWF ∶ Ag,VWF ∶ CB/VWF ∶ Ag were lower than that of southern population group(t=6.373,P=0.001;t=2.902,P=0.005),and there was no significant difference in VWF∶Rco between the two groups (t 1.598,P=0.115).⑥The VWF values of 8 standard plasma samples were in accordance with the known diagnosis.Conclusions A more integrate plasma VWF levels of healthy Chinese people were obtained for the first time by using fully automatic and standardized testing instruments.It was also found that ABO blood group and region had a significant impact on the level of VWF,while the age and gender had no significant effect.%目的 采用全自动标准化方法检测正常人血管性血友病因子(VWF)各指标并分析其影响因素.方法 采用全自动、标准化检测仪及配套试剂测定70名正常人VWF抗原(VWF∶Ag)、VWF瑞斯托霉素辅因子活性(VWF∶Rco)、VWF胶原结合试验(VWF∶CB)、凝血因子FⅧ活性(FⅧ∶C),比较年龄、ABO血型、性别及地域因素对正常人VWF各指标的影响.对英国国家生物标准与检定所(NIBSC)确认的8个标准血浆样本(2名正常人、6例2型VWD)进行VWF各项指标检测.结果 ①70名正常人血浆VWF∶Ag为(95.4+44.9)％,VWF∶ Rco为(105.9±35.4)％,VWF∶CB为(89.8±28.4)％;VWF∶Rco/VWF∶Ag比值为1.18+0.25,VWF∶CB/VWF∶Ag比值为1.03±0.29.②年龄≥30岁组与＜30岁组比较,血浆VWF各指标差异均无统计学意义(P＞0.05).③O型血组VWF∶Rco、VWF∶CB低于非O型血组(t=2.074,P=0.042;t=3.949,P=0.001),VWF∶Ag、VWF∶Rco/VWF∶Ag、VWF∶CB/VWF∶Ag比值差异无统计学意义(P＞0.05).④男、女性组VWF各指标差异无统计学意义(P＞0.05).⑤北方(淮河以北地区)组VWF∶Ag、VWF∶CB高于南方(苏州地区)组(t=4.525,P=0.001;t=3.214,P=0.002),VWF∶Rco/VWF∶Ag、VWF∶CB/VWF∶Ag比值低于南方组(t=6.373,P=0.001;t=2.902,P=0.005).VWF∶Rco差异无统计学意义(t=1.598,P＞0.115).⑥8个标准血浆样本各项VWF指标检测结果符合已知诊断.结论 通过使用全自动标准化检测设备首次获得较完整的正常人血浆VWF水平检测数据;ABO血型和地域因素对VWF水平有显著影响,而年龄和性别的影响不显著.
