Nuclear

摘要： As a young engineer in the power plant department of Brown Boveri,Dr.Schulten had the idea to design nuclear power stations without major risk.The following requirements must be accomplished:ŸA negative temperature coefficient had to avoid an MCA(Maximum Credible Accident);ŸCeramic materials for core construction and fuel elements;ŸA homogenous mixture of nuclear fuel and graphite had to be able to use uranium and thorium as breeding material;ŸThe produced high temperature heat shall be the basis for production of electricity,drinking water,hydrogen,etc.;ŸA relatively simple plant,which could be operated in developing countries,to cogenerate electricity and heat;ŸHelium used as cooling gas.

摘要： Accidents in high-tech organisations are often triggered by a concatenation of human and system anomalies and errors, proving destructive to life, property and the environment. Urgent attention is required to minimize such events by training workers in high-risk organisations and ensuring adequate levels of Non-Technical Skills (NTS) training to counter related risks within the spectrum of their daily tasks. Organisational learning becomes equally relevant when industries are inclined towards becoming learning organisations by encouraging and promoting learning to manage safety. A comparative assessment is drawn by examining current practices in aviation and in the oil and gas sectors. The online survey was used to gather primary data, as well as interviewing 15 safety experts across the three sectors and another 15 safety experts recruited as focus groups to establish if NTS and organisational learning are used in safety management. Our sample comprised health and safety experts from the nuclear (n = 124, 54%), aviation (n = 59, 25%), and oil and gas sectors (n = 49, 21%). Findings revealed that the nuclear sector has not fully and officially acknowledged the use of NTS to train workers. The nuclear sector should look inwardly at how safety is managed since there is limited evidence of formal knowledge or techniques for transferring lessons to staff on NTS, which has proven to be a major critical “ingredient” in safety management in high-risk organisations.

摘要： Protein and protein interactions play important roles in many biological processes and are responsible for carrying out the function of biological regulatory network in living organisms. Previous study indicated that Banana bunchy top virus (BBTV) coat protein (CP) interacted with BBTV nuclear shuttle protein (NSP). However, the protein and protein interaction and the binding affinity of CP and NSP in Babuvirus are remaining unclear. In this study, the CPs and NSPs proteins of BBTV, Abaca bunchy top virus (ABTV) and Cardamom bushy dwarf virus (CBDV) were used for bioinformatic analysis. The binding free energy and the dissociation constant of the possible interaction proteins were tested in PPA-Pred2, and the results confirmed CP interaction with NSP in Babuvirus. The study will help us to understand the interaction between viral protein and viral protein, and the pathogenesis mechanism of Babuvirus in host plants.

摘要： Hepatocyte nuclear factor 4-alpha(HNF4α)is a highly conserved member of nuclear receptor superfamily of ligand-dependent transcription factors that is expressed in liver and gastrointestinal organs(pancreas,stomach,and intestine).In liver,HNF4αis best known for its role as a master regulator of liver-specific gene expression and essential for adult and fetal liver function.Dysregulation of HNF4αexpression has been associated with many human diseases such as ulcerative colitis,colon cancer,maturity-onset diabetes of the young,liver cirrhosis,and hepatocellular carcinoma.However,the precise role of HNF4αin the etiology of these human pathogenesis is not well understood.Limited information is known about the role of HNF4αisoforms in liver and gastrointestinal disease progression.There is,therefore,a critical need to know how disruption of the expression of these isoforms may impact on disease progression and phenotypes.In this review,we will update our current understanding on the role of HNF4αin human liver and gastrointestinal diseases.We further provide additional information on possible use of HNF4αas a target for potential therapeutic approaches.

摘要： Tip60 is a specific member of MYST (Moz-Ybf2/Sas3-Sas2-Tip60) family of nuclear histone acetyltransferases (HAT). It is essential for cellular survival, differentiation, and metabolism. A putative canonical NLS motif between the chromo domain and the zinc finger of Tip60 was identified. Here we show evidence that Tip60 is associated with importin α as its substrate and transported from cytoplasm to the nucleus. Pull down assay revealed that Tip60 was physically associated with importin α both in vivo and in vitro. Confocal microscopic observation showed that Tip60 and importin α were co-localized with each other. The localization of Tip60 to the nuclear and its interaction with importin α was disrupted when its putative NLS motif for binding to importin α was mutated (219RKRK222 → 219AAAA222). However, attachment of this putative NLS motif to a cytoplasmic protein (YAP 1-210 fragment) promoted its nuclear localization. Based on transient transfection, Tip60 NLS motif mutant showed a substantial reduction in self-acetylation, HAT activity, and apoptotic ability whereas wild type Tip60 did not show such reduction. Taken together, our results demonstrate that importin α transports Tip60 from the cytoplasm to the nucleus through binding to the putative NLS motif of Tip60 for its tumor suppressing function.

摘要： The concentration of Radon in mines varies tremendously according to the country rock, type of mineralization and area. Ventilation is also an important factor. The absence of ventilation in mines tends to allow a higher concentration of Radon to build up. This is very dangerous for the miners work inside. In this present work, the radon gas concentration is practically measured in closed uranium prospect mine located at Gabal (G.) Gattar. CR-39 solid state nuclear track detector technique is used. It is found that the radon concentration is around 80 kBq m-3 and an effective ventilation rates should be applied if there will be further works in the future.

摘要： BACKGROUND Hepatocellular carcinoma(HCC) is one of the most common malignant tumors with high mortality-to-incidence ratios. Nuclear factor erythroid 2-like 3(NFE2 L3), also known as NRF3, is a member of the cap ‘n' collar basic-region leucine zipper family of transcription factors. NFE2 L3 is involved in the regulation of various biological processes, whereas its role in HCC has not been elucidated.AIM To explore the expression and biological function of NFE2 L3 in HCC.METHODS We analyzed the expression of NFE2 L3 in HCC tissues and its correlation with clinicopathological parameters based on The Cancer Genome Atlas(TCGA) data portal. Short hairpin RNA(shRNA) interference technology was utilized to knock down NFE2 L3 in vitro. Cell apoptosis, clone formation, proliferation, migration,and invasion assays were used to identify the biological effects of NFE2 L3 in BEL-7404 and SMMC-7721 cells. The expression of epithelial-mesenchymal transition(EMT) markers was examined by Western blot analysis.RESULTS TCGA analysis showed that NFE2 L3 expression was significantly positively correlated with tumor grade, T stage, and pathologic stage. The qPCR and Western blot results showed that both the mRNA and protein levels of NFE2 L3 were significantly decreased after shRNA-mediated knockdown in BEL-7404 and SMMC-7721 cells. The shRNA-mediated knockdown of NFE2 L3 could induce apoptosis and inhibit the clone formation and cell proliferation of SMMC-7721 and BEL-7404 cells. NFE2 L3 knockdown also significantly suppressed the migration, invasion, and EMT of the two cell lines.CONCLUSION Our study showed that shRNA-mediated knockdown of NFE2 L3 exhibited tumor-suppressing effects in HCC cells.

摘要： This work presents the results of the Radon (220Rn and 222Rn) measurements made with 486 chips of CR-39 (Allyl Dyglicol Poly Carbonate) MASL? bared detectors, in a reticular mode distribution, inside of a cellar with average indoor radon concentration 862 ± 49 Bq/m3. The exposure time was 3 months, in microclimate condition of constant temperature, humidity, barometric pressure and no airflow. After these 3 months, all the detectors were chemically etched in KOH 6.25 M solution at 60°C ± 1°C for 18 hours, following a very well established protocol for indoor radon survey by the Dosimetry Applications Laboratory of the Physics Institute of the National Autonomous University of Mexico, and later read automatically by CADIS (Counting Automatically Digital Image System). The results show that each one of the nine measured planes is not homogeneous presenting important differences of indoor radon concentration values. Specifically, the Radon (220Rn and 222Rn) concentration levels vary for each measured point within the cellar. It is a very important observation to consider for the calculation of dose and radiological risk.

摘要： The structure and chemical durability in the vitreous part of the system 60P2O5-2Cr2O3-xFe2O3-(38 - x)Na2O phosphate glasses (with 3 ≤ x ≤ 33 mol%) were investigated using various techniques such as IR spectroscopy, X-ray diffraction and M?ssbauer spectroscopy. The presence of Cr2O3 and the increase of Fe2O3 at the expense of Na2O in the glass network lead to a large number of covalent and rigid Fe-O-P and Cr-O-P bonds. The infrared and XRD spectra indicate a radical change of structure and show that the increase of the Fe2O3 content favors the depolymerization of the vitreous network towards pyrophosphate chains. The presence of Cr2O3 in the glass seems to favor the covalent Cr-O-P bonds linked to the most probable cyclic metaphosphate chains. However, when the Fe2O3 content increases (≥23 mol%), its impact on the glass network is stronger than that of Cr2O3. The infrared and XRD spectra indicate a radical change of structure and show that the increase in Fe2O3 content favors the depolymerization of the vitreous network to short pyrophosphate chains. The results of M?ssbauer spectroscopy indicate the presence of both Fe (III) and Fe (II) ions which occupied more or less deformed octahedral sites. The growth of the glass transition temperature (Tg) with the increase of the iron oxide in the vitreous network, leads to an improvement of the glass rigidity. This explains the decrease of the ionic radius of the iron and the reinforcement of the interconnection of the chains of vitreous networks. The structure of sodium-chromium-iron phosphate glasses can be considered largely as pyrophosphate units linked to ferric and ferrous ions in octahedral or deformed octahedral coordination. The dissolution rate is 200 times lower than that of the silicate glasses.