• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>14th ICC cereal and bread congress and forum on fats amp; oils : Science and technology innovation for healthy cereals amp; oils : book of abstracts >Stable Isotope Dilution Assay for the Accurate Determination of Mycotoxins in Maize by UHPLC-MS/MS
【24h】

Stable Isotope Dilution Assay for the Accurate Determination of Mycotoxins in Maize by UHPLC-MS/MS

机译：UHPLC-MS / MS稳定同位素稀释法准确测定玉米中的真菌毒素

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A fast,easy-to-handle and cost-effective analytical method for 11 mycotoxins currently regulated in maize and other cereal-based food products in Europe was developed and validated for maize.The method is based on two extraction steps using different acidified acetonitrile-water mixtures.Separation is achieved using ultrahigh-performance liquid chromatography (UHPLC) by a linear water-methanol gradient.After electrospray ionisation,tandem mass spectrometric detection is performed in dynamic multiple reaction monitoring mode.Since accurate mass spectrometric quantification is hampered by matrix effects,uniformly [13 C]-labelled mycotoxins for each of the 11 compounds were added to the sample extracts prior to UHPLC-MS/MS analysis.%欧洲目前针对玉米及其他谷类食物,要求控制11种霉菌毒素的含量,为此开发出了一种便于操作且具有成本效率的分析方法,而且通过玉米得到了验证.此方法基于两个萃取步骤,而这两个步骤采用了不同的经酸化的乙腈-水混合液.分离是通过线性水-甲醇梯度超高效液相色谱法(UHPLC)完成的.在电喷雾电离之后,以动态多反应监测模式进行串联质谱检测.由于基质效应会妨碍精密的质谱定量分析,在进行UHPLC-MS/MS分析前,统一向样品萃取物加入分别与11种化合物相对应的[13C]-标记霉菌毒素.在萃取之前以及之后,以霉菌毒素加入空白玉米样品取得方法性能参数,此试验采用三个平行样,针对6个水平进行.双重萃取后两步萃取总所有目标分析物(包括伏马菌素)回收率在97％到111％之间.
机译：A fast,easy-to-handle and cost-effective analytical method for 11 mycotoxins currently regulated in maize and other cereal-based food products in Europe was developed and validated for maize.The method is based on two extraction steps using different acidified acetonitrile-water mixtures.Separation is achieved using ultrahigh-performance liquid chromatography (UHPLC) by a linear water-methanol gradient.After electrospray ionisation,tandem mass spectrometric detection is performed in dynamic multiple reaction monitoring mode.Since accurate mass spectrometric quantification is hampered by matrix effects,uniformly [13 C]-labelled mycotoxins for each of the 11 compounds were added to the sample extracts prior to UHPLC-MS/MS analysis.%欧洲目前针对玉米及其他谷类食物,要求控制11种霉菌毒素的含量,为此开发出了一种便于操作且具有成本效率的分析方法,而且通过玉米得到了验证.此方法基于两个萃取步骤,而这两个步骤采用了不同的经酸化的乙腈-水混合液.分离是通过线性水-甲醇梯度超高效液相色谱法(UHPLC)完成的.在电喷雾电离之后,以动态多反应监测模式进行串联质谱检测.由于基质效应会妨碍精密的质谱定量分析,在进行UHPLC-MS/MS分析前,统一向样品萃取物加入分别与11种化合物相对应的[13C]-标记霉菌毒素.在萃取之前以及之后,以霉菌毒素加入空白玉米样品取得方法性能参数,此试验采用三个平行样,针对6个水平进行.双重萃取后两步萃取总所有目标分析物(包括伏马菌素)回收率在97％到111％之间.

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号