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首页> 外文会议>3rd international symposium on dairy cow nutrition and milk quality proceedings >Detection of the effects of dietary change on rumen proteolytic and cellulolytic bacteria by quantitative real-time PCR
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Detection of the effects of dietary change on rumen proteolytic and cellulolytic bacteria by quantitative real-time PCR

机译：定量实时PCR检测饮食变化对瘤胃蛋白水解和纤维素分解细菌的影响

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IntroductionThe ruminants harbor complex symbiotic microbiota which is responsible for the breakdown of various dietary nutritive components (Metha et al.,2009).The microbiota is highly responsive to changes in diet and feeding regimen (Stewart et al.,1988).It has been reported that the relative abundance of bacterial populations markedly changed with different dietary concentrate to forage ratio or forage type (Tajima et al.,2001;Vlaemincket al.,2006;Methaet al.,2009;Kong et al.,2010;Petri et al.,2012).However there is limited information on how diet affects the composition and distribution of the rumen bacterial community.Techniques of molecular microbial ecology provide an opportunity to quantify these ruminal species with great sensitivity and precision (Tajima et al.,2001;Klieveet al.,2003;Denman et al.,2006;Stevenson et al.,2007).The present study was conducted to quantify the population of rumen proteolytic and cellulolytic bacteria of Chinese Hostein cows fed forage based diets by real-time PCR.
机译：简介反刍动物具有复杂的共生菌群，可导致各种膳食营养成分的分解（Metha等人，2009），该微生物群对饮食和喂养方式的变化具有高度响应性（Stewart等人，1988）。报道指出，不同日粮精料与饲草比例或饲草类型不同，细菌种群的相对丰度显着变化（Tajima等，2001; Vlaemincket等，2006; Methaet等，2009; Kong等，2010; Petri等（2012年）。然而，关于饮食如何影响瘤胃细菌群落组成和分布的信息很少。分子微生物生态学技术提供了一个机会，可以以极高的灵敏度和精确度对这些瘤胃物种进行定量（Tajima等人，2001； 2007）。 Klieveet等人，2003; Denman等人，2006; Stevenson等人，2007）。本研究旨在定量研究饲喂饲喂日粮的中国荷斯坦奶牛的瘤胃蛋白水解和纤维素分解细菌的数量。通过实时PCR。

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  • 会议地点 Beijing(CN)
  • 作者

    X.L.Hu; J.Q.Wang; S.G.Zhao; J.W.Zhao; D.Jin; D.P.Bu; L.Y.Zhou;

  • 作者单位

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

    State Key Laboratory of Animal Nutrition, Institute of Animal Science, Chinese Academy of Agriculture Sciences, Beijing 100193, P.R.China;

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  • 正文语种 eng
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