...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Current Microbiology: An International Journal >Use of real-time PCR technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo
【24h】

Use of real-time PCR technique in studying rumen cellulolytic bacteria population as affected by level of roughage in swamp buffalo

机译：实时PCR技术在研究水牛粗饲料水平对瘤胃纤维素分解细菌种群的影响

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

A real-time polymerase chain reaction approach was used in this study to determine the population of major ruminal bacterial species (Fibrobacter succinogenes, Ruminococcus albus, and Ruminococcus flavefaciens) in digesta and rumen fluid of swamp buffalo (Bubalus bubalis). Four rumen-fistulated, male swamp buffalo were randomly assigned according to a 4 x 4 Latin square design to evaluate the effect of the urea-treated rice straw (roughage source)-to-concentrate ratio on cellulolytic bacterial distribution. Animals were fed roughage-to-concentrate (R:C) ratios of 100:0, 75:25, 50:50, and 25:75, respectively. At the end of each period, rumen fluid and digesta were collected at 0 h and 4 h post-morning-feeding. It was found that feeding urea-treated rice straw solely increased these three cellulolytic bacteria numbers up to 2.65 x 10(9) and 3.54 x 10(9) copies per milliliter for F. succinogenes, 5.10 x 10(7) and 7.40 x 10(7) copies per milliliter for R. flavefaciens, and 4.00 x 10(6) and 6.00 x 10(6) copies per milliliter for R. albus in rumen fluid and digesta, respectively. The distribution of the three cellulolytic bacteria species in digesta were highest at 3.21 x 10(9), 4.55 x 10(7), and 4.56 x 10(6) copies per milliliter for F. succinogenes, R. flavefaciens, and R. albus, respectively. Moreover, at 4 h post-morning-feeding, the populations of the three cellulolytic bacteria were higher than found at 0 h post-morning-feeding. It is most notable that F. succinogenes were the highest in population in the rumen of swamp buffalo and cellulolytic bacteria mostly adhered to feed digesta in the rumen.
机译：在这项研究中使用实时聚合酶链反应方法来确定沼泽水牛（Bubalus bubalis）的消化液和瘤胃液中主要瘤胃细菌种类（琥珀酸纤维杆菌，黄连球菌和黄连球菌）的种群。根据4 x 4拉丁方形设计，随机分配了四个瘤胃f缩的雄性水牛，以评估尿素处理的稻草（粗饲料来源）与浓缩物的比例对纤维素分解细菌分布的影响。给动物饲喂粗饲料与精矿的比率（R：C）为100：0、75：25、50：50和25:75。在每个时期的末期，在喂奶后0小时和4小时收集瘤胃液和消化物。发现饲喂尿素处理过的稻草会使琥珀酸镰刀菌增加这三种纤维素分解细菌的数量分别达到每毫升2.65 x 10（9）和3.54 x 10（9）拷贝，5.10 x 10（7）和7.40 x 10黄曲霉（R. flavefaciens）的每毫升拷贝数为（7）拷贝，瘤胃液和消化液中的白杨的拷贝数分别为每毫升4.00 x 10（6）和6.00 x 10（6）拷贝。三种细菌分解酶在消化菌中的分布最高为：琥珀酸短杆菌基因，黄曲霉和黄腐霉菌分别为每毫升3.21 x 10（9），4.55 x 10（7）和4.56 x 10（6）拷贝。 ， 分别。此外，在早晨喂食后4小时，三种纤维素分解细菌的种群高于早晨喂食后0小时。最值得注意的是，琥珀酸杆菌在沼泽水牛瘤胃中的种群最多，而纤维素分解细菌主要附着在瘤胃的饲料中。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号