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首页> 外文会议>Academic Conference of Asian Society of Traditional Veterinary Medicine; 200610; Beijing(CN) >Simultaneous determination of the content of Baicalin and Chlorgenic acid in Yinhuang injection by HPLC
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Simultaneous determination of the content of Baicalin and Chlorgenic acid in Yinhuang injection by HPLC

机译：高效液相色谱法同时测定银黄注射液中黄ical苷和绿原酸的含量

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摘要

To develop a method for simultaneously determining content of Baicalin and Chlorgenic acid in Yinhuang injection by HPLC. Method: ODS-C18 (150mm × 4.6mm, 5μm) and Gradient Elution Method. The mobile phase was methanol-0.5%KDP. Methanol-0.5%KDP (20:80) at 0.0lmin; methanol-0.5% KDP (80:20) at 0.01 min; methanol-0.5% KDP (20:80) at 11.01min. The detecting wavelength was at 316nm. Chromatographic column temperature was 40℃, and flow rate was 1/min. Results. There was a good linear relationship between the content of Chlorgenic acid and absorption area value with range of 20～60μg, recovery ratio was 99.51%, RSD=1.72%.There was a good linear relationship between the content of Baicalin and absorption area value with range of 10～30μg, recovery ratio was 99.78%, RSD=1.07%.Conclusion. The method is rapid, simple and accurate, and can be used for controlling the quality of the product.
机译：建立一种同时测定银黄注射液中黄ical苷和绿原酸含量的方法。方法：ODS-C18（150mm×4.6mm，5μm）和梯度洗脱法。流动相为甲醇-0.5％KDP。甲醇-0.5％KDP（20:80）在0.0lmin;在0.01分钟内使用甲醇-0.5％KDP（80:20）；在11.01min处加入甲醇-0.5％KDP（20:80）。检测波长为316nm。色谱柱温度为40℃，流速为1 / min。结果。在20〜60μg范围内，绿原酸的含量与吸收面积值呈良好的线性关系，回收率为99.51％，RSD = 1.72％。回收率为10〜30μg，回收率为99.78％，RSD = 1.07％。该方法快速，简便，准确，可用于控制产品质量。

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