Refractive index changes during germination of Bacillus subtilis spores

机译：枯草芽孢杆菌孢子萌发过程中的折射率变化

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Light transmission measurements in the wavelength range of 400 to 1000 nm were performed on Bacillus subtilis spores at periodic time intervals after heat-shock induced activation. The Gaussian ray approximation, using a concentric sphere model for the spore coat and spore core, was used to calculate the scattering cross-section of the spores. Analysis of transmission spectra determined that the refractive index of the spore core was 1.515. In the three hours following heat shock, the core refractive index decreased to 1.39, and subsequently remained constant. During the measurements, the spore radius increased from 0.38 μm to 0.6 μm. The results were confirmed by phase contrast microscopy.

机译：在热激诱导的活化之后，以周期性的时间间隔在枯草芽孢杆菌孢子上进行400至1000 nm波长范围内的透光率测量。高斯射线近似，使用同心球体模型用于孢子皮和孢子核，来计算孢子的散射截面。透射光谱分析确定孢子芯的折射率为1.515。热冲击后的三个小时内，纤芯折射率降至1.39，随后保持恒定。在测量过程中，孢子半径从0.38μm增加到0.6μm。结果通过相差显微镜证实。

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来源
《Advanced Biomedical and Clinical Diagnostic Systems III; Progress in Biomedical Optics and Imaging; vol.6 no.7》|2005年|P.326-329|共4页
会议地点 San JoseCA(US)
作者
A. Katz; A. Alimova; M. Xu; E. Rudolph; P. Gottlieb; J. C. Steiner; R. R. Alfano;
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作者单位

Institute for Ultrafast Spectroscopy Lasers The City College of New York, Convent Ave. 137 St., New York, NY 10031;

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会议组织
原文格式 PDF
正文语种 eng
中图分类光、激光生物医学;
关键词
spore detection; light scattering; spore refractive index; bio-defense;

机译：孢子检测;光散射;孢子折射率;生物防御;

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1. Analysis of the Dynamics of a Bacillus subtilis Spore Germination Protein Complex during Spore Germination and Outgrowth [J] . Anthony J. Troiano, Jingqiao Zhang, Ann E. Cowan, Journal of bacteriology . 2015,第2期

机译：枯草芽孢杆菌孢子萌发蛋白复合物在孢子萌发和生长过程中的动力学分析
2. A soluble protein is immobile in dormant spores of Bacillus subtilis but is mobile in germinated spores: implications for spore dormancy. [J] . Cowan AE, Koppel DE, Setlow B, Proceedings of the National Academy of Sciences of the United States of America . 2003,第7期

机译：可溶性蛋白在枯草芽孢杆菌的休眠孢子中不可移动，但在发芽孢子中可移动：对孢子休眠具有影响。
3. Germination Protein Levels and Rates of Germination of Spores of Bacillus subtilis with Overexpressed or Deleted Genes Encoding Germination Proteins [J] . Kerry-Ann V. Stewart, Xuan Yi, Sonali Ghosh, Journal of bacteriology . 2012,第12期

机译：枯草芽孢杆菌的萌发蛋白水平和孢子萌发率与过表达或缺失的编码萌发蛋白的基因
4. The different pathways of spore germination and inactivation of Bacillus subtilis under high pressure and elevated temperatures [C] . Kai Reineke, Isabel Doehner, Daniel Baier, International congress on engineering and food . 2011

机译：高压下枯草芽孢杆菌的孢子萌发和枯灭性的不同途径和升高的温度
5. Identification of a new protein involved in Bacillus subtilis spore germination and study of its gene expression, localization and cross-linking into the spore coat. [D] . Ragkousi, Katerina. 2004

机译：鉴定一种新的参与枯草芽孢杆菌孢子萌发的蛋白质，并研究其基因表达，定位和交联到孢子皮中。
6. Analysis of the Dynamics of a Bacillus subtilis Spore Germination Protein Complex during Spore Germination and Outgrowth [O] . Anthony J. Troiano Jr., Jingqiao Zhang, Ann E. Cowan, 2015

机译：枯草芽孢杆菌孢子萌发蛋白复合物在孢子萌发和生长过程中的动力学分析
7. Germination of Individual Bacillus subtilis Spores with Alterations in the GerD and SpoVA Proteins, Which Are Important in Spore Germination▿ [O] . Wang, Guiwen, Yi, Xuan, Li, Yong-qing, 2011

机译：枯草芽孢杆菌单个芽的萌发以及GerD和SpoVA蛋白的改变，这在孢子萌发中很重要▿
8. High Pressure Germination of Bacillus subtilis Spores with Alterations in Levels and Types of Germination Proteins. [R] . Doona, C. J., Ghosh, S., Feeherry, F. F., 2014

机译：枯草芽孢杆菌孢子的高压萌发与萌发蛋白质的水平和类型的改变。

Refractive index changes during germination of Bacillus subtilis spores

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