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首页> 外文会议>Biophotonics South America >Fluorescent Liposomes to Probe How DOTAP Lipid Concentrations Can Change Red Blood Cells Homeostasis
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Fluorescent Liposomes to Probe How DOTAP Lipid Concentrations Can Change Red Blood Cells Homeostasis

机译：荧光脂质体可探索DOTAP脂质浓度如何改变红细胞稳态

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摘要

Liposomes have been used to deliver DNA, drugs and, more recently, nanoparticles such as quantum dots, into living cells. Their electrostatic interaction with cell's surface (negatively charged) can lead to membrane destabilization and/or fusion, facilitating intracellular release of those compounds. Nevertheless, cationic lipids can modify living cells homeostasis, depending on their concentration. In this study, we observed that the DOTAP cationic lipid concentrations influence the red blood cells (RBCs) homeostasis. We used fluorescent fusogenic liposomes composed by three lipids: DOPE, DOTAP and DPPE-Rhodamine (1:0.1/0.3/0.5/0.8/1:0.1 mM respectively), varying DOTAP from 0.1 to 1 mM. To probe liposomes ability to fuse with cells, RBCs (1% in saline) were utilized. Liposomes were characterized by zeta potential, dynamic light scattering (DLS), fluorescence and transmission electron microscopy. Their interaction with RBCs was evaluated by fluorescence microscopy and flow cytometry. Zeta potential results showed that, from 0.1 to 1 mM concentration, the charge increases, due to the addition of DOTAP. Liposomes' diameter does not vary significantly when more DOTAP was added, except for the one containing 0.1 mM of DOTAP, according to DLS results. Flow cytometry and microscopy analysis showed that for all DOTAP' concentration applied, the liposomes were capable to label RBCs. However, as higher the amount of DOTAP in liposomes, the more harmful they were to cells. Thus, the results showed that it is possible to use lower concentrations of DOTAP keeping the fusogenic liposomes's ability and cell homeostasis. This is important to guarantee a greater efficiency in the delivery of nanoparticles or other active samples into cells.
机译：脂质体已被用于将DNA，药物以及最近的纳米颗粒（例如量子点）递送到活细胞中。它们与细胞表面的静电相互作用（带负电荷）可导致膜不稳定和/或融合，从而促进这些化合物在细胞内的释放。然而，阳离子脂质可以根据其浓度改变活细胞的体内稳态。在这项研究中，我们观察到DOTAP阳离子脂质浓度会影响红细胞（RBC）的稳态。我们使用由三种脂质组成的荧光融合脂质体：DOPE，DOTAP和DPPE-若丹明（分别为1：0.1 / 0.3 / 0.5 / 0.8 / 1：0.1 mM），DOTAP在0.1到1 mM之间变化。为了探测脂质体与细胞融合的能力，使用了RBC（在盐水中为1％）。脂质体的特征在于ζ电势，动态光散射（DLS），荧光和透射电子显微镜。通过荧光显微镜和流式细胞术评估了它们与红细胞的相互作用。 Zeta电位结果表明，由于添加了DOTAP，从0.1到1 mM的浓度，电荷增加。根据DLS结果，当添加更多DOTAP时，脂质体的直径不会发生明显变化，除了包含0.1 mM DOTAP的脂质体。流式细胞仪和显微镜分析表明，对于所有应用的DOTAP'浓度，脂质体都能够标记RBC。但是，脂质体中DOTAP的含量越高，它们对细胞的危害就越大。因此，结果表明可以使用较低浓度的DOTAP保持融合脂质体的能力和细胞稳态。这对于确保将纳米颗粒或其他活性样品输送到细胞中具有更高的效率非常重要。

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  • 来源
    《Biophotonics South America》|2015年|953139.1-953139.7|共7页
  • 会议地点 Rio de Janeiro(BR)
  • 作者

    Anna L. L. Matos; Goreti Pereira; Beate S. Santos; Adriana Fontes;

  • 作者单位

    Biophysics and Radiobiology Department, Federal University of Pernambuco, Av. Moraes Rego S/N, 50670-901 Recife, PE, Brazil;

    Pharmaceutical Sciences Department, Federal University of Pernambuco, Av. Artur de Sa, S/N, 50740-120 Recife, PE, Brazil;

    Pharmaceutical Sciences Department, Federal University of Pernambuco, Av. Artur de Sa, S/N, 50740-120 Recife, PE, Brazil;

    Biophysics and Radiobiology Department, Federal University of Pernambuco, Av. Moraes Rego S/N, 50670-901 Recife, PE, Brazil;

  • 会议组织
  • 原文格式 PDF
  • 正文语种 eng
  • 中图分类
  • 关键词

    Liposomes; DOTAP; Red blood cells; flow cytometry; fluorescence microscopy;

    机译：脂质体; DOTAP;红细胞;流式细胞仪荧光显微镜;

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