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首页> 外文会议>Conference on Biophotonics South America >Fluorescent Liposomes to Probe How DOTAP Lipid Concentrations Can Change Red Blood Cells Homeostasis
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Fluorescent Liposomes to Probe How DOTAP Lipid Concentrations Can Change Red Blood Cells Homeostasis

机译：荧光脂质体探测DOTAP脂质浓度如何改变红细胞稳态

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Liposomes have been used to deliver DNA, drugs and, more recently, nanoparticles such as quantum dots, into living cells. Their electrostatic interaction with cell's surface (negatively charged) can lead to membrane destabilization and/or fusion, facilitating intracellular release of those compounds. Nevertheless, cationic lipids can modify living cells homeostasis, depending on their concentration. In this study, we observed that the DOTAP cationic lipid concentrations influence the red blood cells (RBCs) homeostasis. We used fluorescent fusogenic liposomes composed by three lipids: DOPE, DOTAP and DPPE-Rhodamine (1:0.1/0.3/0.5/0.8/1:0.1 mM respectively), varying DOTAP from 0.1 to 1 mM. To probe liposomes ability to fuse with cells, RBCs (1% in saline) were utilized. Liposomes were characterized by zeta potential, dynamic light scattering (DLS), fluorescence and transmission electron microscopy. Their interaction with RBCs was evaluated by fluorescence microscopy and flow cytometry. Zeta potential results showed that, from 0.1 to 1 mM concentration, the charge increases, due to the addition of DOTAP. Liposomes' diameter does not vary significantly when more DOTAP was added, except for the one containing 0.1 mM of DOTAP, according to DLS results. Flow cytometry and microscopy analysis showed that for all DOTAP' concentration applied, the liposomes were capable to label RBCs. However, as higher the amount of DOTAP in liposomes, the more harmful they were to cells. Thus, the results showed that it is possible to use lower concentrations of DOTAP keeping the fusogenic liposomes's ability and cell homeostasis. This is important to guarantee a greater efficiency in the delivery of nanoparticles or other active samples into cells.
机译：已经使用脂质体用于将DNA，药物和最近，纳米颗粒如量子点递送到活细胞中。它们与细胞表面（带负电）的静电相互作用可导致膜不稳定和/或融合，促进这些化合物的细胞内释放。然而，阳离子脂质可以根据浓度修饰活细胞稳态。在这项研究中，我们观察到DOTAP阳离子脂质浓度影响红细胞（RBCS）稳态。我们使用了三种脂质组成的荧光致密性脂质体：涂料，DOTAP和DPPE-罗丹明（分别为1：0.1 / 0.3 / 0.5 / 1：0.1mm），从0.1至1mm之间变化的DOTAP。探测脂质体与细胞保险丝的能力，使用RBC（盐水中1％）。通过Zeta电位，动态光散射（DLS），荧光和透射电子显微镜表征脂质体。通过荧光显微镜和流式细胞术评估它们与RBC的相互作用。 Zeta电位结果表明，由于添加DOTAP，电荷增加了0.1至1mm浓度，电荷增加。根据DLS结果，除了加入更多DOTAP时，脂质体直径不会显着变化。除了含有0.1mm Dotap的DOTAP之外。流式细胞术和显微镜分析表明，对于施加的所有DOTAP浓度，脂质体能够标记RBC。然而，随着脂质体中的DOTAP量较高，它们对细胞的有害更有害。因此，结果表明，可以使用较低浓度的DOTAP，保持沉重的脂质体的能力和细胞稳态。这对于保证将纳米颗粒或其他活性样品的递送更高的效率是重要的。

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