• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文会议>Conference on Novel Optical Instrumentation for Biomedical Applications; Jun 24-25, 2003; Munich, Germany >SoftX -ray H olographic Tom ography for B iological Specim ens
【24h】

SoftX -ray H olographic Tom ography for B iological Specim ens

机译：用于生物标本的SoftX射线全息层析成像

获取原文
获取原文并翻译 | 示例
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In this paper, we presant some experimental resultson X -ray holography, holographic tom ography, and a new holographic tomography method called pre-amplified holographictom ography is proposed. Due to the shorter wavelength and the larger penetration depths, X -rays provide the potential of higher resolutionin in aging techniques, and have the ability to in ageintact, living, hydtated cells without slicing, dehydration, chemical fixation orstain. Recently, using X -ray souroe in N ational Synchrotron Radiation Laboratory in Hefei, we have successfully perfarmed some soft X-ray holography experin entson biological specimen. The specimens used in the experim ents was the garlicdove epiderm is, we gottheirX -ray hologram , and then reconstructed them by com puterprograms, the feature of the cellwalls, the nuclei and some cytop lasm were clearly resolved. However, there still exist some problems in realization of practical 3D microscopic in aging due to the near-unity refiactive index of the malter. There is no X -nay optics having a sufficient highnum erical aperture to achieve a depth resolution that is com parable to the transverse resolution. On the other hand, com puter tomography needs arecord of hundreds of views of the test object at different angles forhigh. resolution. This is because the num ber of views required for a densely packed object is equal to the objectiadius divided by the desired depth resolution. Clearly, it is in practical for a radiation-sensitive biological specinen. M oreover, the X -ray diffiaction effect makes projection data blur, this badly degrades the resolution of the reconstructed in age. Inorder to observe 3D structure of the biological specinens, M oN ulty proposed anew method for 3D in aging calledd "holographic tomography (HT)" in which several holograms of the specin enare recoided from various illum ination directions and combined in the reconstruction step. This permits the specinens to be sampled over a wide range of spatial frequencies to in prove the depth resolution, In NSRL, we performed soft X-ray holographic tom ography experm ents. The specimen was the spider filam ents and PM M A as recording medium .By 3D CT reconstruction of the projection data, thiee din ensional density distribution of the specinen was obtained. Also, we developed a new X -ray holographic tom ography mhod called pre-am plified holographic tom ography. The method permits a digitalreal-time 3D reconstruction with high-resolution and a sim ple and com pact experin ental setup aswell.
机译：本文介绍了一些X射线全息照相术，全息层析照相术的实验结果，并提出了一种称为预放大全息照相术的新型全息照相术。由于较短的波长和较大的穿透深度，X射线在老化技术中提供了更高的分辨率，并且能够在不切片，脱水，化学固定或染色的情况下使完整的，活的，水合的细胞老化。最近，我们在合肥国家同步辐射实验室使用X射线源，成功地对一些生物X射线全息照相技术进行了研究。实验中所用的标本是大蒜鸽表皮，我们得到了其X射线全息图，然后用计算机程序对其进行了重建，清楚地分辨了细胞壁，细胞核和一些细胞瘤的特征。但是，由于麦芽的近乎统一的折射率，在实现实用的3D显微镜时效方面仍然存在一些问题。没有X射线光学器件具有足够大的数字孔径以实现与横向分辨率相当的深度分辨率。另一方面，计算机断层扫描需要记录不同角度的测试对象的数百个视角。解析度。这是因为密集包装的对象所需的视图数等于对象偏差除以所需的深度分辨率。显然，它对辐射敏感的生物样本是实用的。更重要的是，X射线衍射效应使投影数据模糊，这严重降低了重建年龄的分辨率。为了观察生物标本的3D结构，M oNulty提出了一种新的3D老化方法，称为“全息层析成像（HT）”，其中从各种照度方向重新排列了几张该种全息图，并在重建步骤中进行了组合。这样可以在很宽的空间频率范围内对样本进行采样，以证明深度分辨率。在NSRL中，我们进行了软X射线全息层析成像实验。标本为蜘蛛丝，PM M A为记录介质。通过投影数据的3D CT重建，获得标本的三维密度分布。此外，我们开发了一种新的X射线全息断层摄影方法，称为pre-am修正全息断层摄影。该方法允许以高分辨率进行数字实时3D重建，并且简单，紧凑的实验设置也是如此。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号