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首页> 外文会议>Conference on Optical Diagnostics of Living Cells Ⅴ, Jan 23-25, 2002, San Jose, USA >Optical Micromanipulation and Analysis of Single Cells on a Microchip Platform
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Optical Micromanipulation and Analysis of Single Cells on a Microchip Platform

机译：微平台上的光学显微操作和单细胞分析

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摘要

Ongoing efforts to engineer a system capable of selecting and labeling single cells using optical micromanipulation tools and performing electrophoretic separation on the contents of a single cell using the "lab-on-a-chip" format are presented. At the heart of this design, are channels with 10 μm diameter cross-sections, etched using a molecular fluorine laser. Individual cells are moved on the microchip using optical tweezers. These single cells are brought into contact with a liposome containing fluorescent tags. The liposome and cell are fused using optical scissors; resulting in a cell with labeled components. This cell is lysed using the optical scissors, and high voltage is applied to separate the contents. This design will allow us to directly look at protein and mRNA expression from a single cell without amplifying the contents of interest, as well as to obtain the population averages and their variations from the analysis of a sufficient number of individual cells.
机译：提出了正在进行的工程设计系统的努力，该系统能够使用光学显微操作工具选择和标记单个细胞，并使用“芯片实验室”格式对单个细胞的内容物进行电泳分离。该设计的核心是采用分子氟激光蚀刻的直径为10μm的通道。单个细胞使用光镊在微芯片上移动。使这些单细胞与含有荧光标签的脂质体接触。用光学剪刀将脂质体和细胞融合；导致细胞带有标记的成分。使用光学剪刀裂解该细胞，并施加高压以分离内容物。这种设计将使我们能够直接查看单个细胞中的蛋白质和mRNA表达，而无需扩增感兴趣的内容，并且可以通过分析足够数量的单个细胞来获得种群平均值及其变异。

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