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首页> 外文会议>Conference on Optics in Tissue Engineering and Regenerative Medicine; 20080120-21; San Jose,CA(US) >Application of polarization OCT in tissue engineering
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Application of polarization OCT in tissue engineering

机译：极化OCT在组织工程中的应用

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For tissue engineering of load-bearing tissues, such as bone, tendon, cartilage, and cornea, it is critical to generate a highly organized extracellular matrix. The major component of the matrix in these tissues is collagen, which usually forms a highly hierarchical structure with increasing scale from fibril to fiber bundles. These bundles are ordered into a 3D network to withstand forces such as tensile, compressive or shear. To induce the formation of organized matrix and create a mimic body environment for tissue engineering, in particular, tendon tissue engineering, we have fabricated scaffolds with features to support the formation of uniaxially orientated collagen bundles. In addition, mechanical stimuli were applied to stimulate tissue formation and matrix organization. In parallel, we seek a nondestructive tool to monitor the changes within the constructs in response to these external stimulations. Polarization-sensitive optical coherence tomography (PSOCT) is a non-destructive technique that provides functional imaging, and possesses the ability to assess in depth the organization of tissue. In this way, an engineered tissue construct can be monitored on-line, and correlated with the application of different stimuli by PSOCT. We have constructed a PSOCT using a superluminescent diode (FWHM 52nm) in this study and produced two types of tendon constructs. The matrix structural evolution under different mechanical stimulation has been evaluated by the PSOCT. The results in this study demonstrate that PSOCT was a powerful tool enabling us to monitor non-destructively and real time the progressive changes in matrix organization and assess the impact of various stimuli on tissue orientation and growth.
机译：对于承重组织（例如骨骼，肌腱，软骨和角膜）的组织工程，生成高度组织化的细胞外基质至关重要。这些组织中基质的主要成分是胶原蛋白，胶原蛋白通常形成高度分层的结构，从原纤维到纤维束的比例不断增加。这些束被订购到3D网络中以承受拉力，压缩力或剪切力。为了诱导有组织的基质的形成并为组织工程特别是肌腱组织工程创建模仿的身体环境，我们制造了具有特征的支架，以支持单轴取向胶原束的形成。另外，施加机械刺激以刺激组织形成和基质组织。同时，我们寻求一种非破坏性的工具来监测构建体中响应这些外部刺激的变化。偏振敏感光学相干断层扫描（PSOCT）是一种无损技术，可提供功能性成像，并具有深入评估组织组织的能力。以这种方式，工程化的组织构建体可以被在线监测，并与通过PSOCT施加的不同刺激相关。在本研究中，我们使用超发光二极管（FWHM 52nm）构造了PSOCT，并产生了两种类型的腱构造。 PSOCT已评估了在不同机械刺激下的基质结构演变。这项研究的结果表明，PSOCT是一种强大的工具，使我们能够无损且实时地监测基质组织的逐步变化，并评估各种刺激对组织方向和生长的影响。

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