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首页> 外文会议>Conference on Optics in Tissue Engineering and Regenerative Medicine >Application of polarization OCT in tissue engineering
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Application of polarization OCT in tissue engineering

机译：极化OCT在组织工程中的应用

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For tissue engineering of load-bearing tissues, such as bone, tendon, cartilage, and cornea, it is critical to generate a highly organized extracellular matrix. The major component of the matrix in these tissues is collagen, which usually forms a highly hierarchical structure with increasing scale from fibril to fiber bundles. These bundles are ordered into a 3D network to withstand forces such as tensile, compressive or shear. To induce the formation of organized matrix and create a mimic body environment for tissue engineering, in particular, tendon tissue engineering, we have fabricated scaffolds with features to support the formation of uniaxially orientated collagen bundles. In addition, mechanical stimuli were applied to stimulate tissue formation and matrix organization. In parallel, we seek a nondestructive tool to monitor the changes within the constructs in response to these external stimulations. Polarization-sensitive optical coherence tomography (PSOCT) is a non-destructive technique that provides functional imaging, and possesses the ability to assess in depth the organization of tissue. In this way, an engineered tissue construct can be monitored on-line, and correlated with the application of different stimuli by PSOCT. We have constructed a PSOCT using a superluminescent diode (FWHM 52nm) in this study and produced two types of tendon constructs. The matrix structural evolution under different mechanical stimulation has been evaluated by the PSOCT. The results in this study demonstrate that PSOCT was a powerful tool enabling us to monitor non-destructively and real time the progressive changes in matrix organization and assess the impact of various stimuli on tissue orientation and growth.
机译：对于负荷组织的组织工程，例如骨，肌腱，软骨和角膜，产生高度有组织的细胞外基质至关重要。这些组织中基质的主要成分是胶原蛋白，其通常形成高度分层结构，随着从纤维到纤维束的增加。这些捆绑包装成3D网络以承受诸如拉伸，压缩或剪切的力量。为了诱导组织基质的形成并为组织工程产生模拟体环境，特别是肌腱组织工程，我们已经制造了具有特征的支架，以支持单轴定向胶原束的形成。此外，应用机械刺激刺激组织形成和基质组织。并行，我们寻求非破坏性工具，以响应这些外部刺激来监测构造内的变化。偏振敏感光学相干断层扫描（PSOCT）是一种提供功能成像的非破坏性技术，并具有评估组织组织深度的能力。以这种方式，可以在线监测工程化组织构建体，并与PSOCT的不同刺激的应用相关。我们在本研究中使用超级发光二极管（FWHM 52nm）构建了一种PSOCT，并产生了两种类型的肌腱构建体。 PSOCT评估了不同机械刺激下的基质结构演化。该研究的结果表明，PSOCT是一个强大的工具，使我们能够监测非破坏性，实时矩阵组织的逐步变化，并评估各种刺激对组织取向和生长的影响。

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