Suppressing nonspecific adsorption of proteins on the single-molecular level

机译：抑制单分子水平上蛋白质的非特异性吸附

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Avoiding nonspecific surface adsorption is a crucial and often challenging issue in many single-molecule studies and analytical applications. In this work, we investigated glass surfaces coated with cross-linking star-shaped polyethylene glycol (4-arm PEG) and demonstrated that this coating can be used for effective suppression of nonspecific protein binding, such as streptavidin. Single-molecule fluorescence images show that only a few molecules remain nonspecifically bound to surfaces treated with protein after sufficient rinsing, i.e. less than to a state-of-the-art BSA coating. Furthermore, different applications for star-shaped PEG-passivated surfaces are shown.

机译：在许多单分子研究和分析应用中，避免非特异性表面吸附是一个至关重要且经常具有挑战性的问题。在这项工作中，我们研究了涂有交联星形聚乙二醇（4-arm PEG）的玻璃表面，并证明该涂层可用于有效抑制非特异性蛋白质结合，例如链霉亲和素。单分子荧光图像显示，在充分漂洗后，只有很少的分子与蛋白处理过的表面保持非特异性结合，即少于最先进的BSA涂层。此外，显示了星形PEG钝化表面的不同应用。

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来源
《Conference on Single Molecule Spectroscopy and Imaging; 20080119-21; San Jose,CA(US)》|2008年|P.ivii|共2页
会议地点 San JoseCA(US)
作者
Nicole Marme; Haemi Lee; Achim Friedrich; Chong-Woo Park; Julie Fiore; David J. Nesbitt; Jens-Peter Knemeyer;
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JILA, National Institute of Standards and Technololgy and University of Colorado, and Department of Chemistry and Biochemistry, University of Colorado, Boulder, CO 80309, USA;

Functional Genome Analysis, German Cancer Research Center, Im Neuenheimer Feld;

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正文语种 eng
中图分类医用物理学;
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1. Raft Polymerization of N,N-Dimethylacrylamide from Magnetic Poly(2-hydroxyethyl methacrylate) Microspheres to Suppress Nonspecific Protein Adsorption [J] . Cao Xueteng, Horak Daniel, An Zesheng, Journal of Polymer Science, Part A. Polymer Chemistry . 2016,第8期

机译：磁性聚甲基丙烯酸2-羟乙酯微球的N，N-二甲基丙烯酰胺筏式聚合以抑制非特异性蛋白质吸附
2. Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy [J] . Schon P, Gorlich M, Coenen MJJ, Langmuir: The ACS Journal of Surfaces and Colloids . 2007,第20期

机译：原子力显微镜研究单分子水平的非特异性蛋白质吸附
3. Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy [J] . Schon P, Gorlich M, Coenen MJJ, Langmuir: The ACS Journal of Surfaces and Colloids . 2007,第20期

机译：原子力显微镜研究单分子水平的非特异性蛋白质吸附
4. Suppressing nonspecific adsorption of proteins on the single-molecular level [C] . Nicole Marme, Haemi Lee, Achim Friedrich, Conference on Single Molecule Spectroscopy and Imaging . 2008

机译：抑制单分子水平上蛋白质的非特异性吸附
5. Controlling nonspecific adsorption of proteins at bio-interfaces for biosensor and biomedical applications. [D] . Dhruv, Harshil D. 2009

机译：控制生物传感器和生物医学应用在生物界面上蛋白质的非特异性吸附。
6. Nonspecific Adsorption of Proteins to Microplates [O] . Sakae Inouye 1973

机译：蛋白质对微孔板的非特异性吸附
7. Nonspecific protein adsorption at the single molecule level studied by atomic force microscopy [O] . Schon P., Gorlich M., Coenen M.J., 2007

机译：原子力显微镜研究单分子水平的非特异性蛋白质吸附
8. Mechanism of Antigen-Antibody Reactions and Similar Nonspecific Reactions Between Proteins and Other Macromolecules [R] . Haurowitz, F. 1966

机译：抗原 - 抗体反应的机制和蛋白质与其他大分子之间的类似非特异性反应

Suppressing nonspecific adsorption of proteins on the single-molecular level

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