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首页> 外文期刊>Journal of Polymer Science, Part A. Polymer Chemistry >Raft Polymerization of N,N-Dimethylacrylamide from Magnetic Poly(2-hydroxyethyl methacrylate) Microspheres to Suppress Nonspecific Protein Adsorption
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Raft Polymerization of N,N-Dimethylacrylamide from Magnetic Poly(2-hydroxyethyl methacrylate) Microspheres to Suppress Nonspecific Protein Adsorption

机译：磁性聚甲基丙烯酸2-羟乙酯微球的N，N-二甲基丙烯酰胺筏式聚合以抑制非特异性蛋白质吸附

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摘要

Nonspecific interaction is a key parameter affecting the efficiency of proteins, nucleic acids or cell separation. Currently, many approaches to introduce antifouling properties to materials have been developed. Among these, surface modification with polymer brushes plays a prominent role. The aim of this study was to synthesize new magnetic microspheres grafted with poly(N,N-dimethylacrylamide) (PDMA) that resist nonspecific protein adsorption. Monodisperse macroporous poly(2-hydroxyethyl methacrylate) (PHEMA) microspheres, 4 mu m in size, were synthesized by a multiple swelling polymerization method. To render the microspheres magnetic, iron oxide was precipitated inside the microsphere pores. Functional carboxyl groups, introduced by the hydrolysis of the 2-(methacryloyl)-oxyethyl acetate (HEMA-Ac) comonomer, were used to react with propargylamine, followed by coupling of a chain transfer agent via an azide-alkyne click reaction. PDMA was grafted from the PHEMA microspheres using reversible addition-fragmentation chain transfer polymerization (RAFT), resulting in surfaces with more than 81 wt % PDMA attached. The successful modification of the microspheres was confirmed by XPS. The magnetic microspheres grafted with PDMA showed excellent antifouling properties as tested in bovine serum protein solutions. (C) 2015 Wiley Periodicals, Inc.
机译：非特异性相互作用是影响蛋白质，核酸或细胞分离效率的关键参数。当前，已经开发了许多将防污性能引入材料的方法。其中，聚合物刷的表面改性起着重要作用。这项研究的目的是合成新的磁性微球接枝聚（N，N-二甲基丙烯酰胺）（PDMA）抵抗非特异性蛋白质吸附。通过多次溶胀聚合法合成了尺寸为4μm的单分散大孔聚甲基丙烯酸2-羟乙酯（PHEMA）微球。为了使微球具有磁性，氧​​化铁沉淀在微球孔内。通过2-（甲基丙烯酰基）-乙酸乙氧基乙酯（HEMA-Ac）共聚单体的水解引入的官能羧基与炔丙基胺反应，然后通过叠氮化物-炔烃点击反应偶联链转移剂。使用可逆的加成-断裂链转移聚合（RAFT）从PHEMA微球接枝了PDMA，导致表面附着了超过81 wt％的PDMA。 XPS证实了微球的成功修饰。如在牛血清蛋白溶液中所测试的，接枝有PDMA的磁性微球显示出优异的防污性能。 （C）2015年Wiley Periodicals，Inc.

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