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首页> 外文会议>Frontiers in biological detection: from nanosensors to systems VI >Integrating SPR-ellipsometry and Electrochemical Measurements for Performance Evaluation of Label-free Thiophene-based Biosensor
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Integrating SPR-ellipsometry and Electrochemical Measurements for Performance Evaluation of Label-free Thiophene-based Biosensor

机译：结合SPR椭圆仪和电化学测量的无标记噻吩基生物传感器的性能评估

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The surface plasmon resonance reflectance changes measured with a circularly polarized ellipsometry and an electrochemical impedance spectroscopy were identified to be able to characterize the critical roles of biomolecules for vastly different biological functions and processes. Throughout the course of this study, interferon-gamma (IFN-γ) was chosen as the biomarker to test and to verify the performance of this newly developed system for Tuberculosis detection. The interactions of IFN-γ with immobilized anti-IFN-γ antibody at various concentrations were interrogated both optically and electrochemically. A semi-conductive linker bis-thiophene was thiolated to ensure the cross-linked monoclonal human IFN-γ antibody got self-assembled onto the gold thin film and form a label-free biosensor. The functional features of the bis-thiophene coated-gold film were characterized by cyclic voltammetry and impedance spectroscopy methods. The association of IFN-y to the bis-thiophene bridging units via antibody-antigen interactions provided the basis for ultrasensitive detection of IFN-γ by tracking the conformation changes in surface-bound protein molecules. The phase shift can be attributed to the average thickness and the real-time index of refraction of the protein layer in different protein layer. Experimental results obtained by impedance spectroscopy and by phase-interrogation SPR showed linear dynamic range. Our experimental results verified that an increase in the concentration of the IFN-γ usually accompanied by phase increase in SPR and an impedance decrease in EIS. These results indicated that our newly developed integrated biosensing system can potentially provide new insight into various conjugate phenomena and interfacial processes for observing molecular conformation changes.
机译：经鉴定，用圆偏振椭圆光度法和电化学阻抗谱法测量的表面等离振子共振反射率变化能够表征生物分子对于极为不同的生物学功能和过程的关键作用。在整个研究过程中，选择干扰素-γ（IFN-γ）作为生物标志物，以测试和验证这种新开发的结核病检测系统的性能。光学和电化学研究了不同浓度的IFN-γ与固定化抗IFN-γ抗体的相互作用。对半导电的联苯双噻吩进行巯基化处理，以确保将交联的单克隆人IFN-γ抗体自组装到金薄膜上，并形成无标记的生物传感器。通过循环伏安法和阻抗谱法表征了双噻吩涂层金膜的功能特性。 IFN-γ通过抗体-抗原相互作用与双噻吩桥联单元的结合，通过跟踪表面结合的蛋白质分子的构象变化，为IFN-γ的超灵敏检测提供了基础。相移可归因于不同蛋白质层中蛋白质层的平均厚度和实时折射率。通过阻抗光谱法和通过相位询问SPR获得的实验结果表明线性动态范围。我们的实验结果证实，IFN-γ浓度的增加通常伴随着SPR的相位增加和EIS的阻抗降低。这些结果表明，我们最新开发的集成生物传感系统可以潜在地为观察分子构象变化的各种共轭现象和界面过程提供新的见解。

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