首页> 外文会议>International Forum on Progress on Post-Genome Technologies(5'IFPT); 20070910-11; Suzhou(CN) >Cellular Interaction base on PEG-mediated Fused Cells On the RT-CESTM System
【24h】

Cellular Interaction base on PEG-mediated Fused Cells On the RT-CESTM System

机译:RT-CESTM系统上基于PEG介导的融合细胞的细胞相互作用

获取原文
获取原文并翻译 | 示例

摘要

A novel method was developed to prepare functionalized magnetic nanoparticles (MNPs), in which SiO2/(PMMA/Fe3O4) magnetic nanoparticles (MNPs) were modified with 3-aminopropyl triethoxysilane to produce a dense amine surface. The amido modified MNPs (NH2-MNPs) were capable of efficient electrostatic capture of genomic DNA. The maximum amount of genomic DNA captured on 10 μg of NH2-MNPs was about 700 ng. PB buffer effectively released the captured DNA. This efficiency was dramatically enhanced by incubation at 80 ℃ and DNA recovery from NH2-MNPs approached 90%. The NH2-MNPs were employed to simplify the isolation of genomic DNA from human whole blood. It is a promising method for high-throughput automated extraction of genomic DNA from whole blood samples at a decreased yield.
机译:开发了一种新的方法来制备功能化的磁性纳米粒子(MNPs),其中SiO2 /(PMMA / Fe3O4)磁性纳米粒子(MNPs)用3-氨丙基三乙氧基硅烷改性以产生致密的胺表面。酰胺基修饰的MNP(NH2-MNP)能够有效地静电捕获基因组DNA。在10μgNH2-MNPs上捕获的最大基因组DNA量约为700 ng。 PB缓冲液有效释放了捕获的DNA。通过在80℃孵育可以大大提高效率,从NH2-MNPs回收的DNA接近90%。 NH2-MNP用于简化从人类全血中分离基因组DNA的过程。这是一种以降低的产量从全血样品中高通量自动提取基因组DNA的有前途的方法。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号