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首页> 外文会议>International Symposium on Supercritical Fluids Tome 1: Natural Products, Process amp; Equipment Development; 20030428-20030430; Versailles; FR >A SFC ANALYSIS METHOD FOR LYCOPENE IN TOMATO EXTRACTS
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A SFC ANALYSIS METHOD FOR LYCOPENE IN TOMATO EXTRACTS

机译：番茄提取物中番茄红素的SFC分析方法

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摘要

Lycopene is a carotinoid naturally present in many fruits and vegetables. It is an important antioxidant providing protection against damage from free radicals, thus reducing risk of various cancers. Extraction of lycopene from tomato by organic solvent or supercritical solvent has been applied to get products with higher lycopene concentration. Accurate and quick measurement of lycopene concentration in the extracts is needed. A method for lycopene analysis in the extract of tomato powders has been established with supercritical fluid chromatography (SFC). With C_(18) as stationary phase, CO_2, CO_2+hexane and CO_2+ethanol as mobile phase, separation lycopene with its degradation product and β -carotene were carried out. The detection of lycopene by high pressure UV detector was set at 472 nm. It was found that separation of lycopene and its degradation product was major task. Therefore measurement of their retention time and separation resolution were studied in various of temperature, 25-50℃, inlet pressure, 15.0-20.0 MPa, pressure drop along column and modifier types and their concentration within 30v%. Lower temperature, higher inlet pressure and higher temperature were found favor better separation and higher efficiency. But higher modifier concentration over 16v% greatly reduces the resolution. Calibration plot and equation was obtained with optimized conditions in linear range of 0.025 mg/ml to 0.1 mg/ml lycopene content. The lycopene content in the extracts by supercritical propane extraction were quantified accurately within 5 minutes.
机译：番茄红素是一种类胡萝卜素，天然存在于许多水果和蔬菜中。它是一种重要的抗氧化剂，可保护人体免受自由基的伤害，从而降低各种癌症的风险。已经应用有机溶剂或超临界溶剂从番茄中提取番茄红素来获得番茄红素浓度较高的产品。需要准确快速地测量提取物中番茄红素的浓度。用超临界流体色谱法（SFC）建立了番茄粉提取物中番茄红素分析的方法。以C_（18）为固定相，以CO_2，CO_2 +己烷和CO_2 +乙醇为流动相，分离番茄红素及其降解产物和β-胡萝卜素。通过高压UV检测器对番茄红素的检测设定为472nm。已发现分离番茄红素及其降解产物是主要任务。因此，研究了它们在25-50℃的温度，入口压力，15.0-20.0 MPa，沿色谱柱和改性剂类型的压降及其浓度在30v％以内的条件下保留时间和分离分辨率的测量。发现较低的温度，较高的入口压力和较高的温度有利于更好的分离和更高的效率。但是高于16v％的较高改性剂浓度会大大降低分辨率。在0.025 mg / ml至0.1 mg / ml番茄红素含量的线性范围内，以优化的条件获得了标定图和方程式。通过超临界丙烷萃取在5分钟内准确定量了萃取物中番茄红素的含量。

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