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Simulated pharmacogenomics exercises for the Cybertory/spl trade/ Virtual Molecular Biology Laboratory

机译：Cyber​​tory / spl贸易/虚拟分子生物学实验室的模拟药物基因组学练习

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The emerging discipline of pharmacogenomics applies genomic technologies to predict individuals' responses to therapeutic drugs based on the genetic sequences of drug targets and enzymes involved in drug metabolism. The first diagnostic test for genotyping two important drug metabolizing enzymes (CYP450 2D6 and 2C19) has been FDA approved. This assay involves PCR amplification and identification of functionally relevant SNPs using a DNA microarray. We have developed a simulation of PCR and microarray analysis of these important enzymes using the open-source Cybertory(TM) Virtual Molecular Biology Laboratory (www. cybertory.org). Genomic sequences are instantiated from these succinct genotype descriptions by substituting allele sequences onto a framework of the full human reference genome. PCR products can be used as probes for microarray hybridization. Using the Cybertory(TM) microarray image generator, we have designed a virtual "SNP chip" to distinguish alleles of CYP450 2D6 based on signal intensities from perfect match and mismatched probe sets.
机译：新兴的药物基因组学学科应用基因组技术，根据药物靶标的遗传序列和参与药物代谢的酶，预测个人对治疗药物的反应。对两种重要的药物代谢酶（CYP450 2D6和2C19）进行基因分型的首次诊断测试已获得FDA批准。该测定涉及使用DNA微阵列的PCR扩增和功能相关SNP的鉴定。我们已经使用开源的Cyber​​tory（TM）虚拟分子生物学实验室（www.cybertory.org）开发了这些重要酶的PCR模拟和微阵列分析。通过将等位基因序列代入完整的人类参考基因组框架中，可以从这些简洁的基因型描述中实例化基因组序列。 PCR产物可用作微阵列杂交的探针。使用Cyber​​tory™微阵列图像生成器，我们设计了一个虚拟的“ SNP芯片”，可根据来自完美匹配和不匹配探针集的信号强度来区分CYP450 2D6等位基因。

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