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首页> 外文会议>ICoMST 2011;International conference of meat science and technology >Effect of breed and diet on subcutaneous beef fat fatty acid indices for enzyme activities and nutritional interest
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Effect of breed and diet on subcutaneous beef fat fatty acid indices for enzyme activities and nutritional interest

机译：品种和饮食对皮下牛肉脂肪脂肪酸酶活性和营养价值的影响

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The effects of breed and diet on several indices of fatty acids involved in MUFA metabolism in subcutaneous fat for 54 Hereford (H), Bradford 1/4 (B 1/4), Bradford 3/8 (B3/8) and Bradford 1/2 (B1/2) steers under three production systems, pasture exclusive (P), pasture and 0.7% corn grain (P+0.7%), and feedlot (F) were determined. Samples of subcutaneous fat were taken at the height of the 11-13 rib and stored at -20°C until analysis. Lipids were extracted using the method of Folch et al (1). The fatty acid methyl esters (FAME) were analyzed on a Chrompack 900 gas chromatograph with a 100 m CP-Sil 88 capillary column. The indices for the activities of A-9 desaturases as well as the elongase a'ctivity were estimated by the ratios of the products of the reaction to the substrates of the reaction. Treatments were compared by analysis of variance using the GML procedure (SAS 8.0 SAS Institute, Inc., Cary, NC). Mean values were compared using the Tukey's test. The ratio MUFA/SFA was affected by diet and breed. F had higher MUFA/SFA ratios than P and P+0.7% and was higher in B1/2, B1/4 and B3/8 compared with H. The ratio 16:1/16:0 was only affected by breed. H was lower than the other breeds. The ratio 18:1/18:0 was only affected by diet. H presented the lowest values. 18:2/18:3 ratio was lower in P compared to F and higher in H and B3/8 compared to B1/2 and B3/8. The CLA concentrations were lower in F and in B3/8.
机译：品种和饮食对54赫里福德（H），布拉德福德1/4（B 1/4），布拉德福德3/8（B3 / 8）和布拉德福德1 /确定了三种生产系统下的2个（B1 / 2）ers牛皮，即牧场专用（P），牧场和0.7％玉米谷物（P + 0.7％）和育肥场（F）。皮下脂肪样品取自肋骨11-13的高度，并保存在-20°C直至分析。使用Folch等人（1）的方法提取脂质。在带有100 m CP-Sil 88毛细管柱的Chrompack 900气相色谱仪上分析脂肪酸甲酯（FAME）。通过反应产物与反应底物的比率来估计A-9去饱和酶活性的指数以及延伸酶的活性。使用GML程序（SAS 8.0 SAS Institute，Inc.，Cary，NC）通过方差分析比较治疗方法。使用图基检验比较平均值。 MUFA / SFA的比例受饮食和品种的影响。 F比P和P + 0.7％具有更高的MUFA / SFA比，与H相比，B1 / 2，B1 / 4和B3 / 8中的F更高。比率16：1/16：0仅受品种影响。 H低于其他品种。 18：1/18：0的比例仅受饮食影响。 H表示最低值。与F相比，P中18：2/18：3的比例要低，而H和B3 / 8中的18：2/18：3的比例要高于B1 / 2和B3 / 8。 F和B3 / 8中的CLA浓度较低。

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