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Electrochemical real-time DNA amplification and detection on a microchip

机译：微芯片上的电化学实时DNA扩增和检测

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In this work, we demonstrate the implementation of a recently developed electrochemical real-time polymerase chain reaction technique on a silicon microchip for simultaneous DNA amplification and detection. The real-time DNA amplification relies on an electroactive DNA intercalator, methylene blue (MB), for electrochemical signal measurements. Compared to other existing electrochemical methods for DNA amplicon analysis, our electrochemical detection method is more advantageous for no requiring probe-modified electrodes, or asymmetric PCR, or solide-phase PCR. Our reaction microchips provide micro PCR environment to promote the efficiency of DNA amplification since the reaction in the microchip wells cost low-volume solution (10μL) and rapid amplification time. In this study, we demonstrate the electrochemical relationship of MB and PCR amplicons with PCR cycles increase by amplifying a fragment of white spot syndrome virus (WSSV) genome DNA. The results of this study prove that the real-time DNA reaction microchip has a great convenience for the rapid, user-friendly, and low-volume real-time PCR assays in the direct detection of WSSV diagnosis.
机译：在这项工作中，我们演示了在硅微芯片上同时开发DNA并同时进行检测的最新开发的电化学实时聚合酶链反应技术的实现。实时DNA扩增依靠电活性DNA嵌入剂亚甲基蓝（MB）进行电化学信号测量。与其他现有的用于DNA扩增子分析的电化学方法相比，我们的电化学检测方法在不需要探针修饰的电极，不对称PCR或固相PCR的情况下更具优势。我们的反应微芯片提供了微PCR环境，可提高DNA扩增的效率，因为微芯片孔中的反应需要小体积的溶液（10μL）和快速的扩增时间。在这项研究中，我们证明了通过扩增白斑综合症病毒（WSSV）基因组DNA片段，MB和PCR扩增子与PCR循环增加之间的电化学关系。这项研究的结果证明，实时DNA反应微芯片在直接检测WSSV诊断中，为快速，用户友好和小批量实时PCR检测提供了极大的便利。

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