首页> 外文期刊>Journal of Biochemical and Biophysical Methods >Immunoassays and sequence-specific DNA detection on a microchip using enzyme amplified electrochemical detection.
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Immunoassays and sequence-specific DNA detection on a microchip using enzyme amplified electrochemical detection.

机译:使用酶放大电化学检测在微芯片上进行免疫测定和序列特异性DNA检测。

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摘要

A CMOS fabricated silicon microchip was used as a platform for immunoassays and DNA synthesis and hybridization. The chip is covered with a biofriendly matrix wherein the chemistries occur. The active silicon chip has over 1000 active electrodes that can be individually addressed for both synthesis of DNA and protein attachment to a membrane on the chip surface. Additionally, the active chip can be further used for the detection of various analytes at the chip surface via digital read out resulting from the redox enzymes on the captured oligonucleotide or antibody.
机译:CMOS制造的硅微芯片被用作免疫测定以及DNA合成和杂交的平台。芯片被生物化学基质覆盖,其中发生化学反应。有源硅芯片具有1000多个有源电极,可以单独寻址这些电极,以合成DNA和蛋白质并附着在芯片表面的膜上。另外,通过捕获的寡核苷酸或抗体上的氧化还原酶产生的数字读出,活性芯片可以进一步用于检测芯片表面的各种分析物。

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