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Composite scaffolds for bone regeneration and infection control

机译：用于骨骼再生和感染控制的复合支架

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To overcome the autologous bone graft limitations, tissue engineering is being explored aiming to develop bone substitutes for reconstructions. Our hypothesis is to develop a composite polylactide (PDLLA) scaffold, coated with collagen, containing Bioglass (BG) and minocycline (MH) - an antibiotic with anti-inflammatory and osteoinductive effects - that would be able to provide an improved regeneration with antimicrobial protection. PDLLA scaffolds were prepared by solvent casting/particulate leaching technique and loaded with MH by adsorption. The morphological characterization was performed by SEM as well as the elemental chemical composition (EDS). Crystalline structure was identified by X-ray diffraction (XRD) and attenuated total reflectance (ATR-FTIR) analysis was assessed to identify the functional groups. Antimicrobial activity against Staphylococcus aureus was evaluated by agar diffusion and the biofilm inhibition formation was observed by SEM analysis. Cellular proliferation and activity were evaluated using human osteoblasts (MG-63 cells). SEM images showed the PDLLA surface with interconnected porous; EDS showed that the BG particles were incorporated into the scaffolds; XRD and ATR evidenced chemical interactions between MH and PDLLA; MH-scaffolds presented antimicrobial activity including biofilm formation reduction. Effects on the cellular proliferation and activity were observed on both samples, without important differences. Overall, results show that PDLLA composite scaffolds show potential as antimicrobial and osteoconductive bone grafts.
机译：为了克服自体骨移植的局限性，正在研究组织工程，目的是开发用于重建的骨替代物。我们的假设是开发一种复合胶原蛋白（PDLLA）支架，该支架上涂有胶原蛋白，其中含有生物玻璃（BG）和米诺环素（MH）（一种具有抗炎和骨诱导作用的抗生素），能够通过抗菌保护提供更好的再生能力。通过溶剂浇铸/微粒浸出技术制备PDLLA支架，并通过吸附负载MH。通过SEM以及元素化学组成（EDS）进行形态表征。通过X射线衍射（XRD）鉴定晶体结构，并通过衰减全反射率（ATR-FTIR）分析来鉴定官能团。通过琼脂扩散评估对金黄色葡萄球菌的抗菌活性，并通过SEM分析观察生物膜抑制形成。使用人成骨细胞（MG-63细胞）评估细胞增殖和活性。 SEM图像显示PDLLA表面具有相互连接的多孔; EDS显示BG颗粒被掺入到支架中。 XRD和ATR证明了MH和PDLLA之间的化学相互作用; MH支架具有抗微生物活性，包括减少生物膜的形成。在两个样品上均观察到对细胞增殖和活性的影响，没有重要差异。总体而言，结果表明，PDLLA复合支架具有作为抗菌和骨传导性骨移植物的潜力。

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