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首页> 外文会议>Annual Conference of the New Zealand Plant Protection >USE OF RIFAMPICIN-RESISTANT BACTERIAL BIOCONTROL STRAINS FOR MONITORING SURVIVAL IN SOIL AND COLONISATION OF PEA SEEDLING ROOTS
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USE OF RIFAMPICIN-RESISTANT BACTERIAL BIOCONTROL STRAINS FOR MONITORING SURVIVAL IN SOIL AND COLONISATION OF PEA SEEDLING ROOTS

机译：利用利福平抗性细菌生物控制菌株监测土壤中生存率及豌豆幼苗根系的殖民

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摘要

Spontaneous rifampicin-resistant strains of two bacterial biocontrol agents, Bacillus subtilis and Paenibacillus polymyxa, with potential to control Aphanomyces euteiches root rot of peas, were used to assess bacterial survival in soil and colonisation of pea seedling roots. The growth and biocontrol characteristics of the rifampicin-resistant strains were similar to the wild type isolates. In two non-sterile soils (Methven and Courtenay), both bacterial strains survived at least 28 days when appliedas inoculum containing high endospore numbers. However, when applied as mostly cells, no bacteria were recovered. Bacteria applied as seed coat formulations colonised both the rhizosphere and rhizoplane of the resulting pea seedlings, with higher recovery from the top 0-4 cm root section compared with the bottom 5-8 cm section. Apart from the top root section for P. polymyxa, where there was no difference in the recovery between the rhizoplane and rhizosphere, recovery of the bacteria was higher in therhizoplane compared with the rhizosphere.
机译：两种细菌生物控制剂，枯草芽孢杆菌和Paenibacillus的抗性抗性菌株，具有控制豌豆的蚜血糖根腐的潜力，用于评估土壤和豌豆幼苗根部的细菌存活。利福平抗性菌株的生长和生物控制特性与野生型分离物相似。在两种非无菌土壤（METHVEN和COURTENAY）中，两种细菌菌株在含有高腹孢子编号的AppliedAnaCum时至少在28天内存活。然而，当主要施用细胞时，没有回收细菌。作为种子涂料制剂施加的细菌归因于所得豌豆幼苗的根际和rhizoplane，与底部5-8cm截面相比，从顶部0-4厘米根部的恢复较高。除了P. polymyxa的顶部剖面外，在rhizoplane和根际之间的恢复没有差异的情况下，与根际相比，细菌的恢复较高。

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