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Identification of DNA markers linked to partial resistance for blast disease in rice across four locations

机译：四个地点鉴定与稻米稻米爆炸症的偏抗性的DNA标记

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One hundred and fourteen doubled haploid plants of IR64/Azucena population were used to identify QTL controlling partial resistance to rice blast disease. The plants were scored for leaf and neck blast reaction in three locations in South India and at IRRI (Philippines). Seedlings were evaluated in nursery for leaf blast and in field for neck blast reaction. The RFLP marker data consisting of 175 DNA markers was used for mapping of QTL (MAPMAKER/QTL, LOD>2.50). Among the several QTL were identified, the major QTL flanked by RG958-RZ816 on chromosome 12 was common in two locations, IRRI, Philippines and Ponnampet, India with a range LOD score of 2.44-8.87, and 10.8% - 27.4% variation. Another QTL on chromosome 5 linked to RZ649-RZ225 markers was identified for leaf blast resistance in Ponnampet location (LOD=2.67, 16.5% variation) and for neck blast resistance in Mudigere location in India (LOD=2.42, 11.1% variation). A major QTL on chromosome 9 linked to Amy3ABC-RG667 markers was identified for neck blast resistance in Bangalore (LOD=2.03, 8.5% variation). The occurence of common QTL could be due to pleiotropic effect of QTL. The tightly linked markers will be fine mapped and used in MAS and in developing isogenic lines.
机译：114个增加了一倍IR64 /阿苏塞纳人口的单倍体植株被用来确定QTL控制水稻稻瘟病局部抵抗力。这些植物进行评分在南印度和国际水稻研究所（菲律宾）三个位置叶瘟和穗瘟反应。幼苗在苗圃叶瘟病和在颈瘟反应场进行了评价。由175个DNA标志物的RFLP标记数据被用于QTL（MAPMAKER / QTL，LOD> 2.50）的映射。其中一些QTL进行了鉴定，主效QTL侧接RG958-RZ816 12号染色体上的两个位置，IRRI，菲律宾和Ponnampet，印度射程LOD值2.44-8.87，而10.8％是常见的 - 27.4％的变化。上连接到RZ649-RZ225标记染色体5 QTL另一个被确定为在Ponnampet位置（LOD = 2.67，16.5％的变化）叶稻瘟病抗性以及在穆迪盖雷位置颈部稻瘟病抗性在印度（LOD = 2.42，11.1％的变化）。上连接到Amy3ABC-RG667标记染色体9所述的主效QTL被确定为在班加罗尔颈部抗稻瘟病（LOD = 2.03，8.5％的变化）。常见的QTL的发生可能是由于QTL的多效性。紧密连锁的标记将被罚款映射和MAS和开发等基因系使用。

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