PULSED-FIELD GEL ELECTROPHORESIS(PFGE) PATTERNS OF ACTINOBACILLUS PLEUROPNEUMON1AE SEROTYPE 2 STRAINS ISOLATED FROM KOREAN SWINE

机译：脉冲场凝胶电泳（PFGE）图案的Actinobacillus pleuropneumon1ae血清型2株从韩国猪分离

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A. pleuropneumoniae is the etiological agent of swine pleuropneumonia, a severe contagious disease causing important economic losses to the swine industry. PFGE separates large DNA fragments created by digestion of total genomic DNA with restriction endonuclease that cut DNA infrequently from several Kb to several Mb which sizes of DNA can not be separated by traditional electrophoresis. Additionally, PFGE can evaluate the genetic relationship between the similar field strains that may be the same inbiotyping and serotyping. In this study, we identified 41 A, pleuropneumoniae isolated from Youngnam and Kyunggi Provinces by polymerase chain reaction and evaluated the genetic relationship of A. pleuropneumoniae serotype 2(24 strains) which is the most prevalent serotype in Korea by pulsed-field gel electrophoresis.

机译：A. Pleuropneumoniae是猪胸膜肺炎的病因，严重传染病，导致猪业的重要经济损失。 PFGE分离通过消化总基因组DNA的大型DNA片段，具有限制性内切核酸酶，其不经常从几kNa到几种Mb，其DNA不能通过传统电泳来分离。另外，PFGE可以评估类似场菌株之间的遗传关系，这些菌株可能具有相同的无酸型和血清型。在这项研究中，我们确定了由聚合酶链反应的杨米和kyunggi省分离的41A，胸膜肠蛋白，并评估了A.Pleuropneumoniae血清型2（24株）的遗传关系，其是脉冲场凝胶电泳是韩国最普遍的血清型。

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《Congress of the International Pig Veterinary Society》|2000年||共1页
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B.H. Kim; J.H. Park; B.Y. Jung; G.J. Cho;
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机译：比较脉冲场凝胶电泳（pFGE）图像中的泳道。

PULSED-FIELD GEL ELECTROPHORESIS(PFGE) PATTERNS OF ACTINOBACILLUS PLEUROPNEUMON1AE SEROTYPE 2 STRAINS ISOLATED FROM KOREAN SWINE

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