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首页> 外文会议>International Symposium of Tissue Engineering for Therapeutic Use >Accumulation of carbonyls accelerates the formation of two advanced glycation endproducts: carbonylstress in uremia
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Accumulation of carbonyls accelerates the formation of two advanced glycation endproducts: carbonylstress in uremia

机译：羰基的积累加速了两种先进的糖钙化封端的形成：尿毒症中的羰基杆菌

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Advanced glycation endproduct (AGE) formation is related to hyperglycemia in diabetes, but not in uremia because plasma AGE levels do not differ between diabetic and nondiabetic hemodialysis patients. The mechanism of this phenomenon remains elusive. We previously suggested that elevation of AGE levels in uremia might result from the accumulation of unknown AGE precursors. In the present study, we evaluate the in vitro generation of two AGEs, pentosidine and carboxymethyllysine (CML). Pentosidine and CML levels were determined by HPLC and GC/MS assay, respectively. Pentosidine and CML were elevated in uremic plasma and were mainly (>95%) albumin bound, demonstrating that their accumulation cannot be attributed to decreased renal clearance of protein-linked pentosidine and CML. Their levels were not correlated with fructoselysine levels and were similar between diabetic and nondiabetic hemodialysis patients, indicating that their increase was not driven by glucose. Plasma samples from normal subjects and nondiabetic hemodialysis patients were incubated under air for several weeks. Both pentosidine and CML rose to a much larger extent in uremic than in control plasma.
机译：先进的糖化封端（年龄）形成与糖尿病中的高血糖有关，但不能在尿毒症中有关，因为糖尿病和非糖尿病血液透析患者之间的血浆年龄水平没有差异。这种现象的机制仍然难以捉摸。我们以前建议尿毒症的年龄水平升高可能是由于未知年龄前体的积累。在本研究中，我们评估了两个年龄，戊糖苷和羧甲基氰基（CML）的体外产生。通过HPLC和GC / MS测定法测定戊胺和CML水平。尿苷血浆和CML升高，主要是（> 95％）白蛋白结合，表明它们的积累不能归因于蛋白质连接的戊胺和CML的肾脏清除。它们的水平与果肉水平无关，糖尿病和非糖尿病血液透析患者之间具有相似，表明它们的增加不是由葡萄糖驱动的。来自正常受试者和非糖尿病血液透析患者的血浆样品在空气下孵育几周。戊胺和CML都升高到尿素中的更大程度，而不是对照等离子体。

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