DEVELOPMENT OF MICROFLUIDIC SHEAR ASSAYS FOR QUANTITATIVE ANALYSIS OF CELL ADHESION

机译：微流体剪切测定的发展，用于定量分析细胞粘附性

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We report a series of microfluidic shear devices for studying cell adhesion on biological substrates. The method provides a tool for performing high throughput experiments with small amounts of sample and number of cells. Furthermore, it allows access to regimes of large adhesion strength that are difficult for conventional cell adhesion assays. We also demonstrate long-term cell culturing and experiments with medium perfusion in such microfluidic devices. These microfluidic devices could provide useful insight for tissue engineering and cell mechanotransduction studies.

机译：我们报告了一系列微流体剪切装置，用于研究生物基材上的细胞粘附。该方法提供了一种用于执行具有少量样品和细胞数的高通量实验的工具。此外，它允许获得难以常见的细胞粘合测定难的粘合强度的规定。我们还展示了在这种微流体装置中具有中灌法的长期细胞培养和实验。这些微流体装置可以为组织工程和细胞机械调制研究提供有用的见解。

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《International conference on miniaturized chemical and biochemical analysis systems》|2002年||共3页
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作者
Hang Lu; Lily Y. Koo; Linda Griffith; Klavs F. Jensen;
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中图分类生物工程学（生物技术）;
关键词
cell adhesion; shear; high throughput;

机译：细胞粘附;剪切;高吞吐量;

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专利

1. Microfluidic shear devices for quantitative analysis of cell adhesion [J] . Lu H, Koo LY, Wang WCM, Analytical chemistry . 2004,第18期

机译：微流体剪切装置用于细胞粘附的定量分析
2. Quantification of the adhesion strength of fibroblast cells on ethylene glycol terminated self-assembled monolayers by a microfluidic shear force assay [J] . Christof Christophis, Michael Grunze, Axel Rosenhahn Physical chemistry chemical physics: PCCP . 2010,第17期

机译：通过微流体剪切力测定法测量乙二醇封端的自组装单层的成纤维细胞粘合强度
3. Mechanism for transition from initial to stable cell-cell adhesion: kinetic analysis of E-cadherin-mediated adhesion using a quantitative adhesion assay. [J] . B Angres, A Barth, W J Nelson Journal of cell biology . 1996,第2期

机译：从初始到稳定的细胞粘附转变的机制：使用定量粘附测定法对E-钙粘蛋白介导的粘附进行动力学分析。
4. DEVELOPMENT OF MICROFLUIDIC SHEAR ASSAYS FOR QUANTITATIVE ANALYSIS OF CELL ADHESION [C] . Hang Lu, Lily Y. Koo, Linda Griffith, International conference on miniaturized chemical and biochemical analysis systems . 2002

机译：微流体剪切测定的发展，用于定量分析细胞粘附性
5. Molecular recognition for the development of microfluidic toxin sensors and adhesion assays. [D] . Frisk, Megan Louise. 2009

机译：用于开发微流毒素传感器和粘附测定的分子识别。
6. Mechanism for transition from initial to stable cell-cell adhesion: kinetic analysis of E-cadherin-mediated adhesion using a quantitative adhesion assay [O] . 1996

机译：从初始到稳定的细胞粘附转变的机制：使用定量粘附测定法对E-钙粘蛋白介导的粘附进行动力学分析
7. Mechanism for transition from initial to stable cell-cell adhesion: kinetic analysis of E-cadherin-mediated adhesion using a quantitative adhesion assay [O] . 1996

机译：从初始到稳定的细胞粘附转变的机制：使用定量粘附测定法对E-钙粘蛋白介导的粘附进行动力学分析

DEVELOPMENT OF MICROFLUIDIC SHEAR ASSAYS FOR QUANTITATIVE ANALYSIS OF CELL ADHESION

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