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Experimental protocol design for The International Space Station Insect Habitat

机译：国际空间站昆虫栖息地的实验协议设计

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The fruitfly, Drosophila melanogaster, is an ideal organism for biological research for several reasons: it is small and relatively simple to culture, has a short generation time, and can be grown in numbers large enough to permit meaningful statistical analysis. Its genetics are well understood and easily manipulated, and a vast stock of mutant and engineered strains exist which facilitate the molecular analysis of almost any biological question. In fact, much of what we currently understand about the basic mechanisms underlying metabolism, development, reproduction, behavior, and aging is derived directly from Drosophila research. For all of these reasons, Drosophila is a logical choice on which to perform basic research into the effects of the spaceflight environment on living systems. The Space Station Insect Habitat is being developed by the Canadian Space Agency in support of this effort. This paper will discuss the challenges encountered in producing a self-contained Insect Habitat capable of not only supporting the growth and development of several generations of Drosophila, but also facilitating the extraction of useful scientific data from sometimes complicated experimental protocols performed with limited crew interaction. One such protocol will be described in detail, illustrating how these logistical problems have been solved, and indicating the sorts of data obtainable in the Habitat and its value to the scientific community.
机译：Fruitfly，Drosophila melanogaster，是一种理想的生物学研究的生物体，培养较小，培养比较简单，并且可以少得多的时间，并且可以在数量大到足以允许有意义的统计分析。其遗传学良好地理解，易于操纵，并且存在巨大的突变和工程菌株，其促进了几乎任何生物问题的分子分析。事实上，我们目前了解新陈代谢，发展，繁殖，行为和衰老的基本机制，直接从果蝇研究中衍生出来。出于所有这些原因，果蝇是一个逻辑选择，用于对生活系统对航天环境的影响进行基础研究。航天站昆虫栖息地是由加拿大空间局开发的，以支持这项努力。本文将讨论生产一种独立的昆虫栖息地遇到的挑战，不仅能够支持几代果蝇的生长和发展，而且还促进了有时复杂的有限船员相互作用的复杂实验方案的有用科学数据的提取。将详细描述一种这样的协议，示出了如何解决这些物流问题，并指示栖息地可获得的数据以及其对科学界的价值。

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