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首页> 外文会议>European Workshop on Equine Nutrition >The effect of three different treatments on the respirable particle content, total viable count and mould concentrations in hay for horses
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The effect of three different treatments on the respirable particle content, total viable count and mould concentrations in hay for horses

机译：三种不同治疗对马马的可吸入颗粒含量，总活计数和霉菌浓度的影响

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Hay, fed to stabled horses can contain high concentrations of respirable particles (RP) (<5 jam), which are the main cause of recurrent airway obstruction (RAO) in horses. This study measured the efficacy of soaking versus steaming at reducing the RPand microbial contamination in hay. Five 30-35 kg bales of barn-stored Lolium perenne hay made June 2011 were treated as follows: for each bale, half was steamed for 40 minutes in a commercial hay steamer, the other half was divided into two 4-6 kg hay nets. One net was kept dry while the other net was soaked in fresh tap water (15 °C) for 10 minutes then drained for 10 minutes. One hundred gram samples from each of the 3 treatments (100 g combined from 5x 20 g sub samples taken in a W shape) were immediately placed into 3 separate pre-labelled (dry, steamed and soaked), sterile plastic bags. The 15 samples (5 dry, 5 steamed and 5 soaked) were prepared as follows: A one gram sub-sample was weighed into a sterile stomacher bag and 79 ml of peptone saline diluent added and the solution processed for 2 minutes to wash particles into the solution. Sequential dilutions were prepared down to 10"4. 1 ml from each dilution was placed onto two 3M1M petrifllms and incubated for 3-5 days at 20, 24 (mould films)and 32 °C (bacteria), before counting using a standard colony counter. RP were determined by shaking under a cyclone sampler, according to the method of Moore-Colyer. Differences between treatments were determined using ANOVA on log transformed data. RP concentrations for dry, soaked and steamed hay were 1,327, 0 and 1.5 (sed = 1.72) geometric mean numbers/litre air/kg DM, respectively. Mould concentrations were 1,174,897, 316,227 and 1,072 (sed 1.97), respectively. Total bacterial counts (TVC) were 21,877,616, 37,153,522 and 83,176 (sed 2.55), respectively. Steaming was the most effective treatment for reducing RP, mould and TVC concentrations. Soaking reduced RP but increased TVC and thus did not improve the hygienic quality of the hay.
机译：干草，喂食稳定的马匹可以含有高浓度的可吸入颗粒（RP）（RP）（<5次果酱），这是经常性气道阻塞（RAO）的主要原因。该研究测量了在减少干草中的RPAND微生物污染时浸泡对蒸汽的疗效。五个30-35公斤大包的谷仓储存的Lolium Perenne Hay在2011年6月进行治疗如下：对于每件捆，一半在商业干草蒸笼中蒸了40分钟，另外一半分为两个4-6千克干草网。一套净保持干燥，而另一个网在新鲜自来水（15°C）中浸泡10分钟，然后排出10分钟。将来自3种处理中的每一个的一百克样品（从5×20g以W形的5×20g副样品组合），立即置于3个单独的预标记（干燥，蒸熟和浸泡），无菌塑料袋中。如下制备15个样品（5次干燥，5次蒸）：将一根克亚样品称为无菌的骨颈袋，并加入79ml蛋白胨盐水稀释剂，并将溶液加工2分钟以洗涤颗粒解决方案。将序列稀释液缩短至10“4。将每次稀释液中1ml置于2个3m1M Petrifllm上，并在使用标准殖民地进行计数之前，在20,24（模具薄膜）和32℃（细菌）中孵育3-5天根据Moore-Colyer的方法，通过在旋风器采样器下振动来确定RP。使用anova在对数转化的数据上测定治疗之间的差异。Rp浓度干燥，浸湿和蒸干草为1,327,0和1.5（SED = 1.72）几何平均数/升空气/千克DM。模具浓度分别为1,174,897,316,227和1,072（SED 1.97）。总细菌计数分别为21,877,616,37,153,522和83,176（SED 2.55）。蒸汽是减少RP，模具和TVC浓度的最有效的治疗方法。浸润减少RP，但TVC增加，因此没有提高干草的卫生质量。

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