The analysis of basic drugs by LC/MS/MS with high pH mobile phases

机译：用高pH移动相的LC / MS / MS分析碱性药物

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Performing chromatographic separations of basic compounds in high pH mobile phases results in extended retention, excellent peak shapes and good efficiency. One possible drawback could be a severe decrease in sensitivity with MS detection, under conditions that suppress analyte ionization in solution. It is common practice to employ volatile weak acids or low pH buffers for enhancing the ionization of basic compounds in positive ionization mode electrospray LC/MS. Thus the LCMS analysis of basic drug compounds in mobile phases of high pH (>analyte pKa+2) could be compromised if ES gas-phase ionization yields were closely linked to acid-base equilibrium in solution.

机译：在高pH移动相中进行碱性化合物的色谱分离导致延长保留，优异的峰形状和良好效率。在抑制溶液中的分析物电离的条件下，一种可能的缺点可能是对MS检测的敏感性的严重降低。常见的做法是使用挥发性弱酸或低pH缓冲剂来提高正电离模式电喷雾LC / MS中的碱性化合物的电离。因此，如果ES气相电离产率与溶液中的酸碱平衡密切相关，则可以损害高pH（>分析物PKA + 2）的移动相的碱性药物化合物的LCMS分析。

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《ASMS Conference on Mass Spectrometry and Allied Topics》|2006年||共2页
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Liming Peng; Ismail Rustamov; Tivadar Farkas;
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中图分类 TB994.5-53;
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The analysis of basic drugs by LC/MS/MS with high pH mobile phases

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