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Analysis of free thiol amino acids and peptides by stable isotope dilution LC-MS

机译：稳定同位素稀释LC-MS对自由硫醇氨基酸和肽的分析

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Thiol-containing amino acids homocysteine (Hcy) and cysteine (Cys) play important roles in regulated various biochemical pathways, whereas the tripeptide glutathione (GSH) is a major determinant of redox status. The ability to quantify each of these thiols is critical for understanding their role in pathophysiological processes (1) . This is a very difficult task because of the relatively low intercellular concentrations of free homocysteine, cysteine and the ease with which they undergo redox cycling with cellular glutathione disulfide and protein disulfides. We have developed a method for the quantification of these thiols using prior derivatization with the specific reagent 4-fluro-7-sulfamoylbenzofurazan (ABD-F) (2). Subsequent quantification using stable isotope dilution methodology makes it possible to analyze these molecules with high precision and accuracy.
机译：含硫醇的氨基酸同型半胱氨酸（HCY）和半胱氨酸（CYS）在规则的各种生化途径中起重要作用，而三肽谷胱甘肽（GSH）是氧化还原状态的主要决定因素。量化这些硫醇中的每一个的能力对于理解其在病理生理过程（1）中的作用至关重要。这是一个非常困难的任务，因为相对较低的自由同型半胱氨酸，半胱氨酸和易于与细胞谷胱甘肽二硫键和蛋白二硫化硫化钠的易氧化还原循环。我们开发了一种使用优先试剂4-Fluro-7-磺酰苯甲酸（ABD-F）（2）的特定试剂4-Fluro-7-巯基（2）的衍生化来定量这些硫醇的方法。随后使用稳定同位素稀释方法进行定量，可以通过高精度和精度分析这些分子。

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