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首页> 外文会议>ASMS Conference on Mass Spectrometry and Allied Topics >A Generic Approach for Absolute Quantitation of the Monoclonal Antibody in Human Serum Using Triple Quadrupole Mass Spectrometry
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A Generic Approach for Absolute Quantitation of the Monoclonal Antibody in Human Serum Using Triple Quadrupole Mass Spectrometry

机译：三重四极孔质谱法的人血清中单克隆抗体绝对定量的通用方法

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摘要

Liquid chromatography-triple quadruple mass spectrometry (LC-MS/MS), a superior analytical technique for quantifying small molecule drugs, has been applied to quantitation of proteins in complex biological matrixes. Its main advantage over ligand binding assays (LBAs) is capability to detect and quantify unique peptides in specific regions of the proteins for overcoming interference from anti-antibody response. In addition, it provides wider linear range and improves assay precision. To develop a generic approach for quantitation of monoclonal antibodies (mAb) in human serum, two unique surrogate tryptic peptides of mAb were detected in multiple reaction-monitoring (MRM) mode. The result shows that LC-MS/MS assay enabled us to achieve lower limit of quantitation of 195 ng/mL and better accuracy within ±10% for the two unique peptides.
机译：液相色谱 - 三倍质谱（LC-MS / MS），一种用于量化小分子药物的优异分析技术，已应用于复杂生物基质中蛋白质的定量。它通过配体结合测定（LBA）的主要优点是能够在蛋白质的特定区域中检测和定量唯一肽，以克服来自抗抗体反应的干扰。此外，它提供更宽的线性范围并提高测定精度。为了开发定量人血清中单克隆抗体（MAB）的通用方法，在多重反应监测（MRM）模式下检测两种独特的替代蛋白酶肽。结果表明，LC-MS / MS测定使我们能够在两个独特的肽的±10％内实现195ng / ml的测量下限，并且在±10％以内。

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