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Quantitative Proteomic Analyses of Serum from Autoimmune Patients

机译:自身免疫患者血清的定量蛋白质组学分析

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1. Quantitative proteomics in serum using MS typically requires depletion. 2. Quantitative proteomics in serum has been performed using MS~E. 3. Approximately 100 proteins could be identified from each plasma sample (~500 ng protein loaded) after depletion. 4. Myositis patient samples are being analyzed in triplicate. 5. A difference in abundance between patients and their healthy siblings as well as the age - matched, unrelated controls are presumably observed for some proteins. Therefore, these are considered biomarkers.
机译:1.使用MS的血清中定量蛋白质组学通常需要耗尽。 2.使用MS〜E进行血清中的定量蛋白质组学。 3.耗尽后,可以从每个血浆样品(〜500ng蛋白质)中鉴定大约100个蛋白质。 4.肌炎患者样品一式三份分析。 5.对于一些蛋白质,可能会观察到患者与健康兄弟姐妹之间丰富的丰富差异,但是对于一些蛋白质,可能会观察到不相关的对照。因此,这些被认为是生物标志物。

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