High Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Analysis of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its Glucuronides in Human Urine

机译：高通量液相色谱 - 串联质谱法分析4-（甲基亚氨基氨基氨基氨基氨基）-1-（3-吡啶基）-1-丁醇（NNAL）及其尿糖苷中的尿糖苷

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A new 96 well plate based high throughput mass spectrometric method for the analysis of free NNAL, NNAL-N-Gluc and NNAL-O-Gluc in small urine volumes from smokers has been developed. The accuracy and precision of the method was determined and the assay was applied to more than 2600 samples for each analyte from a study of smokers from five ethnic groups. In studies where urine volumes are not limited we have used 250 μL of urine with reagents scaled accordingly to produce results with better assay LODs. The basic method should be applicable to studies of smokers, smokeless tobacco users and second hand smoke to further define the utility of these metabolites as potential biomarkers of cancer susceptibility.

机译：已经开发出一种新的96孔板的高吞吐量质谱法，用于分析吸烟者的小尿量中的自由NNAL，NNAL-Gluc和NNAL-o-Gluc。测定该方法的准确性和精度，并将测定应用于每种分析物的超过2600个样品，来自五种族裔的吸烟者。在尿量不受限制的研究中，我们使用了250μl尿液，试剂相应地缩放，以产生具有更好的测定留置的结果。基本方法应适用于吸烟者，无烟烟草用户和二手烟的研究进一步定义这些代谢物的效用作为癌症易感性的潜在生物标志物。

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《American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics》|2013年||共1页
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Steven Carmella; Xun Ming; Andrea Yoder; Elizabeth Vielguth; Stephen S. Hecht;
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1. Rapid quantitation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and its metabolite 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in rat urine using ultra-fast liquid chromatography mass spectrometry (UFLC/MS/MS) [J] . Sepehr Estatira, Woodling Kellie A., Bryant Matthew S. Journal of liquid chromatography and related technologies . 2018,第5a8期

机译：使用的4-（甲基亚氨基氨基）-1-（3-吡啶基）-1-丁酮（NNK）及其代谢物4-（甲基亚氨基）-1-（3-吡啶基）-1-丁醇（NNOL）的快速定量使用超快速液相色谱质谱（UFLC / MS / MS）
2. A new liquid chromatography/mass spectrometry method for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in urine [J] . Bhat S.H., Gelhaus S.L., Mesaros C., Rapid Communications in Mass Spectrometry: RCM . 2011,第1期

机译：尿液中4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁醇（NNAL）的液相色谱/质谱分析新方法
3. Analysis of N- and O-glucuronides of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in human urine. [J] . Carmella StevenG, Le Ka Kyanh, Upadhyaya Pramod, Chemical research in toxicology . 2002,第4期

机译：分析人尿中4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁醇（NNAL）的N-和O-葡萄糖醛酸。
4. High Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Analysis of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its Glucuronides in Human Urine [C] . Steven Carmella, Xun Ming, Andrea Yoder, American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2013

机译：高通量液相色谱 - 串联质谱法分析4-（甲基亚氨基氨基氨基氨基氨基）-1-（3-吡啶基）-1-丁醇（NNAL）及其尿糖苷中的尿糖苷
5. Human pulmonary biotransformation of 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone and impact of genetic polymorphisms on cytochrome P450 1A1-related activities in human lung. [D] . Smith, Graeme Bradford James. 2001

机译：4-（甲基亚硝胺基）-1-（3-吡啶基）-1-丁酮的人肺生物转化以及遗传多态性对人肺中细胞色素P450 1A1相关活性的影响。
6. A new liquid chromatography/mass spectrometry method for 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) in urine [O] . Showket H. Bhat, Stacy L. Gelhaus, Clementina Mesaros, -1

机译：对于4-尿中的新的液相色谱/质谱法（甲基亚）-1-（3-吡啶基）-1-丁醇（NNaL）
7. Development of Liquid Chromatograpy Electrospray Ionization Tandem Mass Spectrometry Methods for Analysis of DNA Adducts of Formaldehyde and Their Application to Rats Treated withN-Nitrosodimethylamine or 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone. [O] . Mingyao Wang, Guang Cheng, Peter W. Villalta, 2008

机译：液相色谱型电离电离串联质谱法，用于分析甲醛DNA加合物及其对用N硝基二甲胺或4-（甲基腈氨基）-1-（3-吡啶基）-1-丁酮处理的大鼠的施用。
8. N-Nitrosodiethylamine and 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone InducedMorphological Transformation of C3H/10T1/2CL8 Cells Expressing Human Cytochrome P450 2A6 [R] . Nesnow, S., Beck, S., Rosenblum, S., 1994

机译：N-亚硝基二乙胺和4-（甲基亚硝基氨基）-1-（3-吡啶基）-1-丁酮诱导表达人细胞色素p450 2a6的C3H / 10T1 / 2CL8细胞的形态转化

High Throughput Liquid Chromatography-Tandem Mass Spectrometry Method for Analysis of 4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanol (NNAL) and its Glucuronides in Human Urine

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