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首页> 外文会议>International conference of meat science and technology >Nuclear Magnetic Resonance Spectroscopy as a Tool to Distinguish between Irradiated and Non-Irradiated Meat
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Nuclear Magnetic Resonance Spectroscopy as a Tool to Distinguish between Irradiated and Non-Irradiated Meat

机译：核磁共振光谱作为区分辐照和非照射肉的工具

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The aim of this study was to assess the usefulness of Proton Nuclear Magnetic Resonance (~1H-NMR) spectroscopy i) to detect the occurrence of the irradiation marker 2-dodecylcyclobutanone (2-dDCB) in irradiated meat, as alternative to the European Standard EN 1785 method, and ii) to differentiate irradiated from non-irradiated meat by coupling spectral data with chemiometrics. !H NMR spectra of 2-dDCB (different concentrations in CDCI_3) and of the lipid fraction (50 mg in 0.8 ml of CDCI_3) extracted from ground beef irradiated at 0, 2.5, 4.5 and 8 kGy were acquired on a Varian INOVA600 NMR spectrometer. Irradiation was performed using a ~(60)Co y-irradiator (1.17-1.33 MeV) on frozen vacuum-packed beef samples (25 g). The data set obtained integrating the whole spectra was statistically elaborated by multivariate analysis. The proton multiplet centred at 2.997 ppm, not overlapped by the intense signals of lipids, was used to identify 2-dDCB. The limit of detection of 2-dDCB was 50 mg/kg meat, much higher than the levels measured in beef irradiated up to 8 kGy. Discriminant analysis applied to NMR spectral data correctly classified 88.9% of originals samples according to the irradiation dose and 81.9% of cross-validated samples. In conclusion, 'H NMR spectroscopy is not suitable to detect 2-dDCB at the levels expected in beef irradiated at doses for commercial purposes. However, coupled with multivariate analysis, it may be considered a promising tool for a rapid detection of irradiated meat.
机译：本研究的目的是评估质子核磁共振（〜1H-NMR）光谱I）检测辐照肉中辐射标志物2-十二烷基环丁酮（2-DDCB）的发生的有用性，欧洲标准的替代品EN 1785方法和II）通过耦合具有化学仪的光谱数据来区分从非辐射肉辐射。在Varian INOVA600 NMR光谱仪上，在Varian Inova600 NMR光谱仪上获得从诸如0,2.5,4.5和8kGy的地牛肉中提取的2-DDCB（CDCI_3中不同浓度）和脂质级分（50mg的50mg CDCI_3）的H NMR光谱。使用〜（60）CO Y辐照器（1.17-1.33meV）在冷冻真空包装牛肉样品（25g）上进行辐射。通过多变量分析统计地阐述了集成整个光谱的数据集。以2.997ppm为中心的质子倍增，不通过脂质的强烈信号重叠，用于识别2-DDCB。 2-DDCB检测限为50mg / kg肉，远高于牛肉照射高达8kGy的水平。根据辐照剂量和81.9％的交叉验证样品正确地分类为NMR光谱数据的判别分析正确分类了88.9％的原始样品。总之，'H NMR光谱不适合于在以商业用量照射的牛肉中预期的水平检测2-DDCB。然而，与多变量分析相结合，它可能被认为是快速检测辐照肉的有希望的工具。

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