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首页> 外文会议>Congress of the International Society for Mushroom Science >Low Temperature Requiring Strains of Paddy Straw Mushroom, Volvariella volvacea and Markers for Their Identification
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Low Temperature Requiring Strains of Paddy Straw Mushroom, Volvariella volvacea and Markers for Their Identification

机译：低温需要水稻吸管蘑菇，volvariella volvacea和标记的衡量标记

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The present study emphasized on developing low temperature requiring strains for fruiting in Volvariella volvacea. A total of 11 strains were evaluated for their fruiting at 22-26°C using chemically treated paddy straw and composted cotton ginning mill waste, followed by studying its relationship with variabilities in ITS sequences, thermal death points and the activities of mycolytic enzymes (chitinase, N-acetyl-β-glucosaminidase and protease). On chemically treated paddy straw, only strains WV-3 of Volvopluteus earlei, Vv-11 and Vv-12 of V. volvacea fruited at bed temperature range of 23-26°C, with the highest yield by WV-3. In bigger scale trials (40 kg compost/bed) at 22-26°C, strain Gw-01 fruited the earliest (11. 93 days post-spawning) and produced the highest yield of 30. 79 kg/100 kg dry substrate on composted cotton ginning mill waste, followed by strains Vv-13 and Vv12. The remaining eight strains gave very low yields. Strain GVv-01 giving the highest yield at low temperature conditions is distinct from low yielding strains in the dendrogram deduced from the ITS sequences. Together with data from a previous study using four strains, the fruiting ability at low temperature was not found to have any correlation with activities of chitinase and N-acetyl-βglucosaminidase recorded on storage of cultures at refrigerated conditions. However, the activity of protease was recorded to be the lowest in strain Vv-11 giving the second highest pinning after strain GVv-01, while highest in strain Vv-09 that produced no fruiting at low temperature conditions. Thermal death point of low temperature requiring strains Gvv-01 and Wv-03 was found to be the lowest, followed by Vv-13 and Vv-11 under dry conditions and it may have some relationship with the temperature requirement of fruiting in these strains. The study has implications in finding low temperature requiring strains for fruiting and the development of markers for their identification.
机译：目前研究强调开发volvariella volvacea中的低温需要菌株的菌株。在22-26℃下使用化学处理的水稻秸秆和堆肥棉花轧机废物评估11种菌株，然后研究其与其序列中的变形性，热死亡点和霉菌酶活性的关系（几丁质酶，N-乙酰-β-葡糖胺酶和蛋白酶）。在化学处理过的水稻秸秆上，仅在床温度范围为23-26°C的VV-11和VV-12的VV-3和VV-12的VV-3和VV-12的菌株。通过WV-3的最高收益率。在22-26°C的更大规模试验（40公斤堆肥/床）中，应变GW-01最早（11. 93天后产卵后11天），并产生30.79kg / 100kg干基质的最高收率堆肥棉花轧轧厂废物，其次是菌株VV-13和VV12。剩余的8个菌株产生了很低的产量。在低温条件下给出最高产率的菌株GVV-01与从其序列推导的树枝图中的低产生菌株不同。与来自先前研究的数据一起使用四种菌株，未发现低温下的结果能力与记录在冷藏条件下培养物储存的几丁质酶和N-乙酰基-β-葡萄糖蛋白酶的活性有任何相关性。然而，将蛋白酶的活性被记录为菌株VV-11中最低的最低菌株GVV-01之后的第二最高钉扎，而在低温条件下没有产生果实的菌株VV-09中的最高。低温的热死亡点需要菌株GVV-01和WV-03是最低的，然后是VV-13和VV-11在干燥条件下，它可能与这些菌株中果的温度要求具有一些关系。该研究在寻找低温方面对需要菌株以及标记的发育进行鉴定，该研究具有含义。

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