• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>Journal of Plant Biochemistry and Biotechnology >Development of molecular and biochemical markers for selecting a potential high yielding strain of paddy straw mushroom (Volvariella volvacea).
【24h】

Development of molecular and biochemical markers for selecting a potential high yielding strain of paddy straw mushroom (Volvariella volvacea).

机译：开发分子和生物化学标记物，以选择潜在的高产稻草菇（Volvariella volvacea）。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Twelve 'normal type' strains of paddy straw mushroom (Volvariella volvacea) varied in their mycelial growth characteristics, and the fast growing strains, OE-215, OE-209 and OE-212 exhibited higher activity of cellulases, but not of laccase and polyphenol oxidase. In protein profiles, out of twelve, two strains, OE-210 and OE-273 varied only at one protein band with respect to its intensity. The four strains, OE-272, OE-140, OE-209 and OE-55 with higher activity of endoglucanase, beta -glucosidase, xylanase and laccase, were also recorded to have higher protein content in their mycelial cultures. The RAPD profiles obtained with 5 OPB series primers varied in different strains and the combined phylogenetic analysis of the RAPD profiles revealed 5 distinct phylogenetic clusters, where in the strains with common origin showed 85% to 95% similarity. The strain, OE-210, with a low intensity protein band in its protein profile also varied in mycelial growth rate on two different media, possessed highest activity of endoglucanase, beta -glucosidase, laccase and polyphenol oxidase and also showed highest dissimilarity of 28% with other strains in phylogenetic analysis. The present study thus, reveals that strains with faster mycelial growth rate, forming aerial mycelia and chlamydospores, also exhibited higher activities of xylanase, exoglucanase, endoglucanase or beta -glucosidase, while simply higher activity of an individual enzyme was not found sufficient to support the mycelial growth and mushroom yield. A relationship between origin of the strains and their phylogenetic belongingness was also recorded, as the strains from different origins formed different clusters in combined phylogenetic analysis.
机译：稻草蘑菇（Volvariella volvacea）的十二个“正常类型”菌株的菌丝体生长特性各不相同，而快速生长的菌株OE-215，OE-209和OE-212表现出较高的纤维素酶活性，而漆酶和多酚则没有。氧化酶。在蛋白质谱中，在十二个菌株中，两个菌株OE-210和OE-273仅在一个蛋白带上相对于其强度发生变化。具有内切葡聚糖酶，β-葡糖苷酶，木聚糖酶和漆酶活性较高的四个菌株OE-272，OE-140，OE-209和OE-55也被记录为在其菌丝体培养物中具有更高的蛋白质含量。用5种OPB系列引物获得的RAPD图谱在不同菌株中有所不同，并且对RAPD图谱的综合系统进化分析显示5种不同的系统进化簇，其中在共同起源的菌株中显示出85％至95％的相似性。 OE-210菌株在蛋白质谱中具有低强度蛋白带，在两种不同培养基上的菌丝体生长速率也不同，具有最高的内切葡聚糖酶，β-葡糖苷酶，漆酶和多酚氧化酶活性，并且也显示出最高的相异性28％与其他菌株进行系统发育分析。因此，本研究表明，菌丝生长速度较快，形成气生菌丝和衣原体孢子的菌株还表现出较高的木聚糖酶，外切葡聚糖酶，内切葡聚糖酶或β-葡萄糖苷酶活性，而单个酶的较高活性不足以支持该酶。菌丝生长和蘑菇产量。还记录了菌株起源与它们的系统发生归属之间的关系，因为在综合系统发育分析中，来自不同起源的菌株形成了不同的簇。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号