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首页> 外文会议>Proceedings of the 18th congress of the International Society for Mushroom Science >Low Temperature Requiring Strains of Paddy Straw Mushroom, Volvariella volvacea and Markers for Their Identification
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Low Temperature Requiring Strains of Paddy Straw Mushroom, Volvariella volvacea and Markers for Their Identification

机译：稻草菇低温菌种的发酵要求及其鉴定标志物

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The present study emphasized on developing low temperature requiring strains for fruiting in Volvariella volvacea. A total of 11 strains were evaluated for their fruiting at 22-26℃ using chemically treated paddy straw and composted cotton ginning mill waste, followed by studying its relationship with variabilities in ITS sequences, thermal death points and the activities of mycolytic enzymes (chitinase, N-acetyl-β-glucosaminidase and protease). On chemically treated paddy straw, only strains WV-3 of Volvopluteus earlei, Vv-11 and Vv-12 of V. volvacea fruited at bed temperature range of 23-26℃, with the highest yield by WV-3. In bigger scale trials (40 kg compost/bed) at 22-26℃, strain Gw-01 fruited the earliest (11. 93 days post-spawning) and produced the highest yield of 30. 79 kg/100 kg dry substrate on composted cotton ginning mill waste, followed by strains Vv-13 and Vv12. The remaining eight strains gave very low yields. Strain GVv-01 giving the highest yield at low temperature conditions is distinct from low yielding strains in the dendrogram deduced from the ITS sequences. Together with data from a previous study using four strains, the fruiting ability at low temperature was not found to have any correlation with activities of chitinase and N-acetyl-βglucosaminidase recorded on storage of cultures at refrigerated conditions. However, the activity of protease was recorded to be the lowest in strain Vv-11 giving the second highest pinning after strain GVv-01, while highest in strain Vv-09 that produced no fruiting at low temperature conditions. Thermal death point of low temperature requiring strains Gvv-01 and Wv-03 was found to be the lowest, followed by Vv-13 and Vv-11 under dry conditions and it may have some relationship with the temperature requirement of fruiting in these strains. The study has implications in finding low temperature requiring strains for fruiting and the development of markers for their identification.
机译：目前的研究重点在于发展低温所需的菌种，以便在旋花菌中结实。使用化学处理过的稻草和堆肥轧花厂废料，评估了11株菌株在22-26℃时的结果，然后研究了其与ITS序列变异性，热死亡点和霉菌分解酶（几丁质酶， N-乙酰基-β-氨基葡萄糖苷酶和蛋白酶）。在经过化学处理的稻草上，只有沃尔沃螺旋藻的WV-3菌株，旋毛弧菌的Vv-11和Vv-12菌株在23-26℃的床温范围内结实，其中WV-3的产量最高。在22-26℃的较大规模试验（40千克堆肥/床）中，菌株Gw-01最早（产卵后11.93天）结实，堆肥时最高产量为30. 79千克/ 100千克干基质轧棉厂废料，其次是菌株Vv-13和Vv12。其余八株菌株的收率很低。在低温条件下能提供最高产量的菌株GVv-01与从ITS序列推导的树状图中的低产量菌株截然不同。连同先前使用4个菌株的研究数据，未发现低温下的结实能力与在冷藏条件下储存的培养物中记录的几丁质酶和N-乙酰基-β-氨基葡萄糖苷酶的活性没有任何相关性。然而，蛋白酶活性被记录为在菌株Vv-11中最低，从而给出了在菌株GVv-01之后第二高的钉扎，而在菌株Vv-09中最高，其在低温条件下没有结果。低温条件下需要菌株Gvv-01和Wv-03的热死点最低，其次是干燥条件下的Vv-13和Vv-11，这可能与这些菌株的结实温度要求有关。这项研究对寻找需要结实的低温菌株和鉴定其标记物具有重要意义。

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