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首页> 外文会议>Joint Conference on Chemistry >Quantitative analysis of Curcuminoid collected from different location in Indonesia by TLC-Densitometry and its antioxidant capacity
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Quantitative analysis of Curcuminoid collected from different location in Indonesia by TLC-Densitometry and its antioxidant capacity

机译：用TLC密度测定法及其抗氧化能力从印度尼西亚不同地点收集的姜黄素的定量分析

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Curcuminoid, consisting of curcumin, demethoxycurcumin and bis demethoxycurcumin, is the active compound in Curcuma longa L. rhizome. It yielded vary depend on the environment and varieties. Curcumin has been known to have a potent antioxidants activity. The present work was conducted to assess the curcuminoid content and antioxidant capacity in the crude extracts of C. longa L. collected from eight different locations in Indonesia. Samples were collected from eight locations including Java island, Sumatera island and Nusa Tenggara island. All samples were extracted using 96% ethanol and analyzed quantitatively using TLC-Densitometry. Antioxidant activity was assessed using diphenylpicrylhydrazyl (DPPH) radical scavenging assay and analyzed at 517 run using a spectrophotometer. Curcuminoid content in C. longa varied among eight different locations (0.53±0.05-5.33±0.12 % w/w). The highest curcumin, demethoxycurcumin and bisdemethoxycurcumin yield were found in the samples from Magetan (5.33±0.12%, 1.54±0.05%, 0.46±0.02% w/w, respectively). In contrast, the lowest curcumin, demethoxycurcumin and bisdemethoxycurcumin yield were found in the sample from Demak (0.53±0.05%, 0.17±0.05%, 0.17±0.05% w/w, respectively). Antioxidant capacity showed similar for all places unless sample from Magetan which exhibited two times lower than other locations. It is apparently curcuminoid content and antioxidant activity varied among places. These results are useful information for curcuminoid standardization method in pharmaceutical products.
机译：由姜黄素，去甲氧基核和双甲氧基杂菌蛋白组成的姜黄素是姜黄中的活性化合物。它产生的变化取决于环境和品种。已知姜黄素具有有效的抗氧化剂活性。进行了本作本作的作品，以评估来自印度尼西亚八个不同地点的C. longa L.的粗提物中的姜黄素含量和抗氧化能力。从包括Java岛，Sumatera岛和Nusa Tenggara岛的八个地点收集样品。使用96％乙醇提取所有样品，并使用TLC密度测定法定量分析。使用二苯基吡啶基酰基（DPPH）自由基清除测定评估抗氧化活性，并在517使用分光光度计分析。 C. Longa中的姜黄素含量在八个不同的位置变化（0.53±0.05-5.33±0.12％w / w）。在Magetan的样品中发现了最高姜黄素，去甲氧杂菌蛋白和双甲氧键产率（分别为5.33±0.12％，分别为1.54±0.05％，0.46±0.02％w / w）。相反，在Demak的样品中发现了最低姜黄素，去甲氧键菌蛋白和双甲氧键产蛋白产量（分别0.53±0.05％，0.17±0.05％，0.17±0.05％w / w）。抗氧化能力与所有地方相似，除非来自Magetan的样品，除非来自其他位置的两倍。它显然是姜黄素含量和抗氧化活性在地方各不相同。这些结果是药品中姜黄素标准化方法的有用信息。

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