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首页> 外文会议>Conference on Smart Biomedical and Physiological Sensor Technology >Electric fields assisted fluorescence enhancement for microRNA biomarker detection in serum samples: strategies for combating cancer, obesity and addiction to opioid
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Electric fields assisted fluorescence enhancement for microRNA biomarker detection in serum samples: strategies for combating cancer, obesity and addiction to opioid

机译：电场在血清样品中辅助荧光增强MicroRNA生物标志物检测：对抗癌症，肥胖和成瘾对阿片类药物的策略

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Cancer, obesity, and opioid abuse pose a combined threat to the well-being of the people in the United States, affectingover 70% and costing more than $250 billion per year in medical expenses. The unavailability of sensing technologiesaddressing the fundamental molecular changes related to disease initiation, progression, and therapeutic interventionsis a critical roadblock for successfully combating these diseases/disorders. Recent clinical studies have shown thatmicroRNA (miRNA) in circulating blood could use as a potential biomarker for combating these diseases/disordersbecause miRNA expression take place first in the biochemical cascade and therefore, miRNA could provide reliableand clinically important information that is superior and appear earlier than other biomarkers. Despite this progress,miRNAs have not yet been translated or utilized in the clinical diagnosis of any disease. This lack of progress ispartially due to the differences among and limitations of various detection technologies, which produce inconsistentand inaccurate results. To address this issue, we have developed a low-cost and disposable device that can detect andquantify target miRNA levels. MiRNA detection is an integrated two-step process that used external electric fields toselectively concentrate fluorophore-labeled target miRNAs in nanoscale metallic hotspots within the device andenhance the fluorescence intensity via multiple metal-fluorophore interactions. This paper demonstrates how externalelectric fields could modulate the radiative decay rate of fluorophore molecules and subsequently enhance thefluorescence intensity.
机译：癌症，肥胖和阿片类药物滥用对美国人民的福祉构成了综合威胁，影响在医疗费用中超过70％，每年花费超过250亿美元。传感技术的不可用解决与疾病启动，进展和治疗干预相关的基本分子变化是成功打击这些疾病/疾病的重要路障。最近的临床研究表明循环血液中的microRNA（miRNA）可以用作对抗这些疾病/疾病的潜在生物标志物因为miRNA表达首先在生化级联中进行，因此，MiRNA可以提供可靠的和临床上的重要信息，比其他生物标志物更早。尽管这一进展，MiRNA尚未在任何疾病的临床诊断中被翻译或使用。这种缺乏进展是部分原因是各种检测技术的差异，产生不一致的检测技术和不准确的结果。要解决此问题，我们开发了一种可以检测和频繁的低成本和一次性设备量化目标miRNA水平。 miRNA检测是一种使用外部电场的集成两步处理在装置内的纳米级金属热点中选择性地浓缩荧光团标记的目标miRNA通过多种金属 - 荧光团相互作用增强荧光强度。本文展示了外部外部电场可以调节荧光团分子的辐射衰减率，随后增强荧光强度。

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