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Microelectrodes integrated cell-chip for drug effects study

机译：微电极集成细胞芯片用于药物效应研究

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Silicon-based microelectrode chips are useful tools for temporal recording of neurotransmitter releasing from neural cells. Both invasive and non-invasive methods are targeted by different group researchers to perform electrical stimulating on neural cells. A microfabricated microelectrodes integrated biochip will be presented in this paper, which describes the dopaminergic cells growing on the chip directly. The dopamine exocytosis can be detected non-invasively from drug incubated dopaminergic cells growing on the chip. The abovementioned silicon-based electrochemical sensor chip has been designed with an electrode array located on the bottom of reaction chamber and each electrode is individually electrical controlled. MN9D, a mouse mesencephalic dopaminergic cell line, has been grown on the surface of the biochip chamber directly. Dopamine exocytosis from the chip-grown MN9D cells was detected using amperometry technology. The amperometric detection limit of dopamine of the biochip microelectrodes was found from 0.06μM to 0.21μM (S/N=3) statistically for the electrode diameters from 10 μm to 90 μm, the level of dopamine exocytosis from MN9D cells was undetectable whithout drug incubation. In contrast, after MN9D cells were incubated with L-dopa, a dopamine precursor, K+ induced dopamine extocytosis was temporally detected. The microelectrodes integrated biochip provides a non-invasive, temporal detection of dopamine exocytosis from dopaminergic cells, and holds the potential for applications in studying the mechanisms of dopamine exocytosis, and drug screening. It also provides a tool for pharmaceutical research and drug screening on dopaminergic cells, extendably to be used for other cell culture and drug effects study.
机译：基于硅基微电极芯片是用于从神经细胞释放神经递质的时间记录的有用工具。侵入性和非侵入性方法都是由不同组研究人员靶向的，以对神经细胞进行电气刺激。本文将介绍一种微型制衡的微电极集成的Biochip，其描述了直接在芯片上生长的多巴胺能细胞。可以从芯片上生长的药物培养的多巴胺能细胞进行非侵入性地检测多巴胺外尿。上述基于硅基电化学传感器芯片设计有位于反应室底部的电极阵列，并且每个电极是单独的电控。 MN9D是一种小鼠脑脑高巴因细胞系，直接在Biochip室的表面上生长。使用Amperometry技术检测来自芯片生长的MN9D细胞的多巴胺外尿精。生物芯片微电极的多巴胺的水平检测限为0.06μm至0.21μm（s / n = 3），对于电极直径为10μm至90μm，来自MN9D细胞的多巴胺卵细胞瘤水平不可检测的次蛋白孵育。相反，在将MN9D细胞与L-DOPA温育之后，在时间检测到多巴胺前体，K +诱导的多巴胺尖滴度。微电极集成Biochip提供了来自多巴胺能细胞的非侵入性，时间检测多巴胺卵尿作用，并具有应用在研究多巴胺外尿量和药物筛选机制的应用。它还为在多巴胺能细胞上提供了药物研究和药物筛选的工具，可伸张用于其他细胞培养和药物效应研究。

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