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Microelectrodes Integrated Cell-Chip for Drug Effects Study

机译：微电极集成细胞芯片用于药物作用研究

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Silicon-based microelectrode chips are useful tools for temporal recording of neurotransmitter releasing from neural cells. Both invasive and non-invasive methods are targeted by different group researchers to perform electrical stimulating on neural cells. A microfabricated microelectrodes integrated biochip will be presented in this paper, which describes the dopaminergic cells growing on the chip directly. The dopamine exocytosis can be detected non-invasively from drug incubated dopaminergic cells growing on the chip. The abovementioned silicon-based electrochemical sensor chip has been designed with an electrode array located on the bottom of reaction chamber and each electrode is individually electrical controlled. MN9D, a mouse mesencephalic dopaminergic cell line, has been grown on the surface of the biochip chamber directly. Dopamine exocytosis from the chip-grown MN9D cells was detected using amperometry technology. The amperometric detection limit of dopamine of the biochip microelectrodes was found from 0.06μM to 0.21μM (S/N=3) statistically for the electrode diameters from 10 μm to 90 μm, the level of dopamine exocytosis from MN9D cells was undetectable whithout drug incubation. In contrast, after MN9D cells were incubated with L-dopa, a dopamine precursor, K~+ induced dopamine extocytosis was temporally detected. The microelectrodes integrated biochip provides a non-invasive, temporal detection of dopamine exocytosis from dopaminergic cells, and holds the potential for applications in studying the mechanisms of dopamine exocytosis, and drug screening. It also provides a tool for pharmaceutical research and drug screening on dopaminergic cells, extendably to be used for other cell culture and drug effects study.
机译：硅基微电极芯片是用于临时记录神经细胞释放神经递质的有用工具。侵入性和非侵入性方法都受到不同小组研究人员的针对，以对神经细胞进行电刺激。本文将介绍一种集成了微加工微电极的生物芯片，该芯片描述了直接在芯片上生长的多巴胺能细胞。可以从在芯片上生长的药物孵育的多巴胺能细胞中无创检测多巴胺胞吐作用。上述基于硅的电化学传感器芯片已经被设计成具有位于反应室底部的电极阵列，并且每个电极都被单独地电控制。 MN9D是一种小鼠中脑多巴胺能细胞系，已直接在生物芯片腔室的表面上生长。使用电流分析技术检测了芯片生长的MN9D细胞的多巴胺胞吐作用。在电极直径从10μm到90μm的情况下，统计发现生物芯片微电极的多巴胺安培检测极限为0.06μM至0.21μM（S / N = 3），在没有药物孵育的情况下，无法检测到MN9D细胞的多巴胺胞吐水平。相反，将MN9D细胞与多巴胺前体L-多巴一起孵育后，会暂时检测到K +诱导的多巴胺胞吐作用。集成了微电极的生物芯片提供了一种非侵入性的，从多巴胺能细胞中暂时检测多巴胺胞吐作用的方法，具有在研究多巴胺胞吐作用机理和药物筛选方面的潜力。它还为多巴胺能细胞的药物研究和药物筛选提供了工具，可广泛用于其他细胞培养和药物作用研究。

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