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Imaging and etching: soft x-ray microscopy on whole wet cells

机译：成像和蚀刻：整个湿细胞上的软X射线显微镜

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We have produced images of whole wet tissue culture cells with the Stony Brook/BNL scanning transmission x-ray microscope (STXM). For fixed cells we have taken images at theoretical resolutions of approximately 50 - 75 nm, and in practice have measured FWHM of features down to near 100 nm, without any exotic image processing. For un-fixed (i.e., initially live) cells we have imaged with 100 nm pixels and measured features down to 250 nm. In order to do this we have developed, tested, and used a wet cell for maintaining fixed or live cells on the STXM stage during imaging. Our design of the wet cell and the culture substrates that go with it make the STXM compatible with almost all standard systems for surface adherent tissue culture. We show some new images of whole wet fixed and unfixed cells, with visible sub-micron features. We also report data that helps to characterize the tissue damage due to x-ray absorption during STXM imaging.
机译：我们用石质Brook / BNL扫描透射X射线显微镜（STXM）产生了整个湿组织培养细胞的图像。对于固定电池，我们已经在大约50-75nm的理论分辨率下拍摄了图像，并且在实践中测量了FHA的特征下降到100nm，而没有任何异国图像处理。对于未固定的（即，最初的Live）单元格，我们已经用100nm像素进行了成像，并测量特征下降到250nm。为了做到这一点，我们已经开发了，测试并使用了用于在成像期间在STXM阶段保持固定或活细胞的湿细胞。我们设计的湿细胞和培养基的培养基，使STXM与几乎所有标准系统兼容的表面粘附组织培养。我们展示了一些全湿固定和未固定的细胞的新图像，具有可见的亚微米特征。我们还报告了有助于在STXM成像期间由于X射线吸收而表征组织损伤的数据。

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