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首页> 外文会议>Conference on biomedical instrumentation based on micro- and nanotechnology >Measuring Liquid Volumes in Sub-nanoliter Wells
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Measuring Liquid Volumes in Sub-nanoliter Wells

机译：测量亚纳米孔井中的液体体积

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We are developing a method for high-throughput screening using arrays of "nanowells" built into a silicon substrate. These wells can serve as bioreactors for studying a variety of biochemical reactions such as the enzymatic activity that occurs in yeast metabolism. For a variety of studies it is important to know the volume of liquid that has been deposited in a given well and/or to monitor the evaporation of the liquid. Using silicon as our substrate means that we can take advantage of the ability to build microelectronics into the wells in order to develop "smart" wells. The wells are manufactured on silicon wafers using conventional photolithography and etching techniques and typical wells measure 200 X 200 X 20 mu m~3 which is a volume of 800 pl. Aluminum electrodes are patterned on the floor of the wells. The floor as well as the electrodes are then covered by an electrical insulation layer. The complex impedance measured through the electrodes is then related to the volume of liquid in the wells. Using fluorescence microscopy as well as interference microscopy to calibrate our system, we can measure liquid volumes with an accuracy of about 5 percent and a resolution better than 1 pl. Real-time monitoring of fluid volumes in a collection of wells is possible by additional on-chip microelectronics which permits multiplexing the measurements over the bioreactor array.
机译：我们正在使用内置在硅衬底中的“纳米孔”阵列开发一种用于高通量筛选的方法。这些孔可以作为生物反应器，用于研究各种生化反应，例如酵母代谢中发生的酶活性。对于各种研究，重要的是要知道在给定孔中沉积的液体的体积和/或监测液体的蒸发。使用硅作为我们的基板意味着我们可以利用将微电子能够建立到井中的能力，以便开发“智能”孔。孔在硅晶片上使用传统的光刻和蚀刻技术制造，典型的孔测量200×200×20μm〜3，其体积为800 pL。铝电极在孔的地板上图案化。然后由电绝缘层覆盖地板以及电极。然后通过电极测量的复杂阻抗与孔中的液体体积有关。使用荧光显微镜以及干扰显微镜来校准我们的系统，我们可以测量液体体积，精度约为5％，分辨率优于1 pl。通过额外的片上微电子可以实时监测井集中的流体体积，这允许在生物反应器阵列上复用测量。

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